Global Patent Index - EP 0012494 A1

EP 0012494 A1 19800625 - A DNA transfer vector, a microorganism modified by said vector and the synthesis of a eucaryotic protein by the modified microorganism.

Title (en)

A DNA transfer vector, a microorganism modified by said vector and the synthesis of a eucaryotic protein by the modified microorganism.

Title (de)

DNS-Transfer-Vektor, durch diesen Vektor modifizierter Mikroorganismus und Synthesis eines eukariotischen Proteins durch den modifizierten Mikroorganismus.

Title (fr)

Vecteur de transfert d'ADN, microorganisme modifié par ce vecteur et synthèse d'une protéine eucariotique par ce microorganisme modifié.

Publication

EP 0012494 A1 19800625 (EN)

Application

EP 79301630 A 19790813

Priority

US 93303578 A 19780811

Abstract (en)

A deoxyribonucleic acid (DNA) transfer vector is provided which comprises a nucleotide sequence coding for a eucaryotic protein joined to a procaryotic control fragment, the respective reading frames being in register, and the respective directions of transcription being the same. By connecting a eucaryotic gene to a gene for a protein normally compartmentalized or protected from intracellular degradation in some manner, the stability of a chimeric protein may by enhanced. A eucaryatic gene may be linked to a gene which has desirable genetic properties. In addition to the DNA transfer vector, the invention also provides a microorganism transformed by the transfer vector, a protein synthesised by such a microorganism, and a method of synthesising a protein.

IPC 1-7

C12N 15/00; C12P 21/02

IPC 8 full level

A61K 38/27 (2006.01); C07K 14/005 (2006.01); C12N 15/09 (2006.01); C07K 14/195 (2006.01); C07K 14/575 (2006.01); C07K 14/61 (2006.01); C07K 14/62 (2006.01); C07K 19/00 (2006.01); C12N 1/20 (2006.01); C12P 19/34 (2006.01); C12P 21/02 (2006.01); C12R 1/19 (2006.01)

CPC (source: EP)

C07K 14/61 (2013.01); C07K 14/62 (2013.01); C07K 2319/00 (2013.01); C07K 2319/75 (2013.01)

Citation (search report)

  • FR 2281426 A1 19760305 - ICI LTD [GB]
  • LU 79174 A1 19780628 - DIGICOURSE INC [US]
  • FR 2384024 A1 19781013 - MAX PLANCK GESELLSCHAFT [DE]
  • EP 0001929 A2 19790516 - GENENTECH INC [US]
  • EP 0001930 A2 19790516 - GENENTECH INC [US]
  • FR 2348971 A1 19771118 - UNIV MINNESOTA [US]
  • DE 2644432 A1 19780330 - RESEARCH CORP
  • SCIENCE, Vol. 198 (4321), 9 December 1977. K. ITAKURA et al. "Expression in Escherichia coli of a Chemically Synthesized Gene for the Hormone Somatostatin", pages 1056-1063. * Figures 1,3; page 1062, 2nd column, 2nd paragraph - end of paper *
  • NATURE, Vol. 275, 12 October 1978. O. MERCEREAU-PUIJALON et al. "Synthesis of an Ovalbumin-like protein by Escherichia coli K12 harbouring a recombinant plasmid" pages 505-510. * Figures 2,3,5 *
  • NATURE, Vol. 275, 19 October 1978, A.C.Y. CHANG "Phenotypic expression in E.coli of a DNA sequence coding for mouse dihydrofolate reductase". pages 617-624. * Figure 1 *
  • NATURE, Vol. 276, 21/28 December 1978. P.H. SEEBURG et al. "Synthesis of Growth hormone by bacteria", pages 795-798. * Whole paper *
  • PROC.NATL. ACAD. SCIE. USA, Vol. 75, No. 8, Aug. 1978, L. VILLA-KOMAROFF et al. "A bacterial clone synthesizing proinsulin", pages 3727-3731. * Whole paper *
  • PROC. NATL. ACAD. SCI. USA, Vol. 76, No. 1, Jan. 1979, D.V. GOEDDEL et al. "Expression in Escherichia coli of chemically synthesized genes for human insulin", pages 106-110. * Figure 1; pages 108-110: Results and discussion *
  • RESEARCH DISCLOSURE, July 1979, No. 183, ref. No. 18309. "The production of interferon by genetic engineering". pages 361-362. * Whole paper *
  • SCIENCE, Vol 196, 17 June 1977 A. ULLRICH et al. "Rat insulin genes: construction of Plasmids containing the coding sequences", pages 1313-1319.
  • NATURE, Vol. 270, 8 December 1977, P.H. SEEBURG et al. "Nucleotide sequence and amplification in bacteria of structural gene for rat growth hormone".

Designated contracting state (EPC)

BE CH DE FR LU NL SE

DOCDB simple family (publication)

EP 0012494 A1 19800625; EP 0012494 B1 19831109; AT 373917 B 19840312; AT A550579 A 19830715; AU 4984379 A 19800424; AU 533035 B2 19831027; CS 219257 B2 19830325; DD 147369 A5 19810401; DE 2966390 D1 19831215; DK 334979 A 19800212; FI 792481 A 19800212; GB 2031434 A 19800423; GB 2031434 B 19830302; GR 71912 B 19830816; IE 48385 B1 19841226; IL 58016 A0 19791230; IL 58016 A 19831031; IT 7925087 A0 19790813; JP S5545395 A 19800331; NZ 191278 A 19821123; PH 22855 A 19890119; PL 217721 A1 19800701; PT 70051 A 19790901; YU 195679 A 19840229; ZA 794172 B 19801231

DOCDB simple family (application)

EP 79301630 A 19790813; AT 550579 A 19790813; AU 4984379 A 19790813; CS 549279 A 19790810; DD 21494879 A 19790813; DE 2966390 T 19790813; DK 334979 A 19790810; FI 792481 A 19790809; GB 7928156 A 19790813; GR 790159805 A 19790809; IE 153379 A 19790809; IL 5801679 A 19790810; IT 2508779 A 19790813; JP 10214579 A 19790810; NZ 19127879 A 19790810; PH 22887 A 19790810; PL 21772179 A 19790811; PT 7005179 A 19790810; YU 195679 A 19790810; ZA 794172 A 19790810