EP 0036737 A2 19810930 - Lactase preparation.
Title (en)
Lactase preparation.
Title (de)
Herstellung von Laktase.
Title (fr)
Production de lactase.
Publication
Application
Priority
US 13303680 A 19800324
Abstract (en)
A process for the release of lactase from yeast cells comprising contacting the cells with an alkyl alcohol or a dialkyl ketone, followed by extraction of the lactase in an aqueous solution at a pH in the range 5.5 to 8.0 is disclosed. Also disclosed is a process for the selective reduction of protease activity in lactase preparations comprising heating an aqueous solution of lactase containing protease impurities in the presence of glycerol. Lactase stability during the heating process can optionally be enhanced by addition of manganous ion.
IPC 1-7
IPC 8 full level
CPC (source: EP US)
C12N 9/2471 (2013.01 - EP US); C12N 9/86 (2013.01 - EP US); C12Y 302/01023 (2013.01 - EP US); C12Y 305/02006 (2013.01 - EP US); Y10S 435/814 (2013.01 - EP US); Y10S 435/911 (2013.01 - EP US); Y10S 435/921 (2013.01 - EP US)
Citation (applicant)
- WENDORF ET AL., J. MILK FOOD TECH., vol. 34, 1971, pages 451
- HEYM, ARCH. BIOCHEM. BIOPHYS., vol. 127, 1962, pages 89
Designated contracting state (EPC)
AT BE CH DE FR GB IT LU NL SE
DOCDB simple family (publication)
EP 0036737 A2 19810930; EP 0036737 A3 19830511; EP 0036737 B1 19860205; AT E17868 T1 19860215; DE 3173692 D1 19860320; DK 129881 A 19810925; DK 167124 B1 19930830; DK 167226 B1 19930920; DK 27093 A 19930310; DK 27093 D0 19930310; JP S56148290 A 19811117; JP S5910191 B2 19840307; JP S6128389 A 19860208; JP S6254471 B2 19871116; US 4329429 A 19820511
DOCDB simple family (application)
EP 81301094 A 19810316; AT 81301094 T 19810316; DE 3173692 T 19810316; DK 129881 A 19810323; DK 27093 A 19930310; JP 3623185 A 19850225; JP 4225881 A 19810323; US 13303680 A 19800324