Global Patent Index - EP 0051593 A1

EP 0051593 A1 19820519 - ISOLATION OF CARBOHYDRATES FROM CELL TISSUE.

Title (en)

ISOLATION OF CARBOHYDRATES FROM CELL TISSUE.

Title (de)

ISOLIERUNG VON KOHLEHYDRATEN AUS ZELLGEWEBEN.

Title (fr)

ISOLATION D'HYDRATES DE CARBONE A PARTIR DE TISSUS CELLULAIRES.

Publication

EP 0051593 A1 19820519 (EN)

Application

EP 81900324 A 19810128

Priority

SE 8000658 A 19800128

Abstract (en)

[origin: WO8102104A1] A process for isolating the carbohydrate fraction (oligosaccharides) from cell tissue with degradation of the glycoconjugates, the cell tissue being treated with a reagent containing or consisting of trifluoroacetic acid anhydride, and the carbohydrates being released under conversion to trifluoracetates, which are optionally detrifluoroacetylated. Starting from erytrocyte membranes oligosaccharides possessing blood group specificity can be recovered, and such oligosaccharides are useful for blood group determination.

Abstract (fr)

Procede d'isolation de la fraction hydrates de carbone (oligosaccharides) a partir de tissus cellulaires avec degradation des glycoconjugues, le tissu cellulaire etant traite avec un reactif contenant ou se composant d'anhydride d'acide trifluoroacetique, les hydrates de carbone etant liberes a la suite de leur conversion en trifluoroacetates, qui sont facultativement detrifluoroacetyles. En partant de membranes erythrocytes, des oligosaccharides possedant une specificite de groupes sanguins peuvent etre recuperes, a partir de tels oligosaccharides et sont utiles pour la determination du groupe sanguin.

IPC 1-7

A61K 31/70; C07H 1/08; C07H 3/06

IPC 8 full level

A61K 31/70 (2006.01); A61K 35/12 (2006.01); A61K 35/18 (2006.01); C07H 1/00 (2006.01); C07H 3/06 (2006.01); C07K 16/34 (2006.01); G01N 33/80 (2006.01)

CPC (source: EP)

A61K 31/70 (2013.01); C07H 3/06 (2013.01); C07K 16/34 (2013.01); G01N 33/80 (2013.01)

Designated contracting state (EPC)

DE FR GB NL

DOCDB simple family (publication)

WO 8102104 A1 19810806; DK 427581 A 19810925; EP 0051593 A1 19820519; IT 1141962 B 19861008; IT 8119379 A0 19810128; JP S57500022 A 19820107; SE 8000658 L 19810729

DOCDB simple family (application)

SE 8100021 W 19810128; DK 427581 A 19810925; EP 81900324 A 19810128; IT 1937981 A 19810128; JP 50055081 A 19810128; SE 8000658 A 19800128