Global Patent Index - EP 0066552 A1

EP 0066552 A1 19821208 - Method for determining the creatinine concentration in biological liquids and reactive for carrying out the method.

Title (en)

Method for determining the creatinine concentration in biological liquids and reactive for carrying out the method.

Title (de)

Verfahren zur Ermittlung der Kreatininkonzentration in biologischen Flüssigkeiten und Reagens zur Durchführung des Verfahrens.

Title (fr)

Procédé pour déterminer la concentration de créatinine dans des liquides biologiques et réactif pour réaliser ce procédé.

Publication

EP 0066552 A1 19821208 (EN)

Application

EP 82830126 A 19820510

Priority

IT 2200981 A 19810528

Abstract (en)

The method for determining the creatinine concentration or contents in biological liquids comprises the steps of transforming enzymatically the creatinine into glycine, acetaldehyde and hydrogen peroxide and proportioning one of these compounds. Advantageously there is proportioned the hydrogen peroxide by titration, or potentiometric, colorimetric or enzymatic procedures. Preferably the enzymatic method is used by catatasis or peroxydasis in the presence of such compounds effective to provide a chromogen compound. The enzymatic transformation of creatinine into the three compounds is carried out by means of creatinineamidohydrolasis, creatineamidohydrolasis and sarcosineoxydasis.

IPC 1-7

C12Q 1/34; C12Q 1/00; G01N 33/70

IPC 8 full level

C12Q 1/34 (2006.01); G01N 33/70 (2006.01)

CPC (source: EP)

C12Q 1/34 (2013.01); G01N 33/70 (2013.01)

Citation (search report)

  • [X] DE 3003490 A1 19800814 - MILLIPORE CORP
  • [YP] EP 0033462 A1 19810812 - ELVI SPA [IT]
  • [X] CHEMICAL AND PHARMACEUTICAL BULLETIN, Vol. 28, No. 12, 1980, Tokyo, T. KINOSHITA et al. "A Fluorophotometric Determination of Serum Creatinine and Creatine using a Creatinineamidohydrolase-Creatineamidinohydrolase-Sarcosine Oxidase-Peroxidase System and Diacetyldichlorofluorescin" pages 3501 to 3506
  • [X] JOURNAL OF ANALYTICAL CHEMISTRY OF U.S.S.R., Vol. 35, No. 8, Part 2, August 1980, New York, I.F. DOLMANOVA et al. "Enzymatic Methods of Analysis" pages 1042 to 1081

Designated contracting state (EPC)

AT BE CH DE FR GB LI NL SE

DOCDB simple family (publication)

EP 0066552 A1 19821208; IT 1137075 B 19860903; IT 8122009 A0 19810528

DOCDB simple family (application)

EP 82830126 A 19820510; IT 2200981 A 19810528