EP 0075444 B1 19921223 - METHODS AND PRODUCTS FOR FACILE MICROBIAL EXPRESSION OF DNA SEQUENCES
Title (en)
METHODS AND PRODUCTS FOR FACILE MICROBIAL EXPRESSION OF DNA SEQUENCES
Publication
Application
Priority
US 30368781 A 19810918
Abstract (en)
[origin: EP0075444A2] Microbial expression of an exogenous polypeptide may be inefficient if the messenger RNA has secondary structure (due to complementarity of spaced regions of the molecule) which impedes interaction with a ribosome. The occurrence of such structure can be predicted. The degeneracy of the genetic code gives freedom to alter the nucleic acid composition of the cDNA to eliminate harmful secondary structure of the mRNA. For example, bovine growth hormone (BGH) is poorly expressed by E. coli transformed with a recombinant plasmid containing the natural BGH gene. The corresponding mRNA has complementary regions at 46-51 and 73-78. Therefore a plasmid pBGH 33-4 was constructed containing a BGH gene whose upstream portion was synthesized (making use of plasmid pHGH 207-1 which contains an efficiently expressible gene for the closely related human growth hormone) so as not to cause the troublesome complementarity. E. coli transformed therewith produced BGH with a yield of better than 10<5> copies per cell.
IPC 1-7
IPC 8 full level
A61K 38/27 (2006.01); C07H 21/04 (2006.01); C07K 14/575 (2006.01); C07K 14/61 (2006.01); C12N 1/00 (2006.01); C12N 1/21 (2006.01); C12N 15/00 (2006.01); C12N 15/09 (2006.01); C12N 15/10 (2006.01); C12P 21/00 (2006.01); C12P 21/02 (2006.01); C12R 1/19 (2006.01); C12R 1/91 (2006.01)
CPC (source: EP)
C07K 14/61 (2013.01); C12N 15/10 (2013.01)
Designated contracting state (EPC)
AT BE CH DE FR GB IT LI LU NL SE
DOCDB simple family (publication)
EP 0075444 A2 19830330; EP 0075444 A3 19841227; EP 0075444 B1 19921223; AT E127518 T1 19950915; AU 3279089 A 19890914; AU 563347 B2 19870709; AU 632282 B2 19921224; AU 8846482 A 19830324; BR 8205453 A 19830823; CA 1340371 C 19990202; DE 3280422 D1 19930204; DE 3280422 T2 19930527; DE 3280471 D1 19951012; DE 3280471 T2 19960404; DK 172897 B1 19990920; DK 413982 A 19830425; ES 515800 A0 19841001; ES 8407329 A1 19841001; GB 2106119 A 19830407; GB 2106119 B 19860521; GB 2147902 A 19850522; GB 2147902 B 19860514; GB 8416356 D0 19840801; IE 53897 B1 19890412; IE 822281 L 19830318; JP 2539775 B2 19961002; JP 2766781 B2 19980618; JP H0776599 A 19950320; JP S5863390 A 19830415; NZ 201918 A 19870430; ZA 826802 B 19830727
DOCDB simple family (application)
EP 82304880 A 19820916; AT 87202220 T 19820916; AU 3279089 A 19890413; AU 8846482 A 19820916; BR 8205453 A 19820916; CA 411650 A 19820917; DE 3280422 T 19820916; DE 3280471 T 19820916; DK 413982 A 19820916; ES 515800 A 19820917; GB 8226403 A 19820916; GB 8416356 A 19840627; IE 228182 A 19820917; JP 16153682 A 19820916; JP 18327894 A 19940804; NZ 20191882 A 19820915; ZA 826802 A 19820916