Global Patent Index - EP 0087238 A1

EP 0087238 A1 19830831 - Am improved method for preparing human insulin from non-human insulin.

Title (en)

Am improved method for preparing human insulin from non-human insulin.

Title (de)

Methode zur Herstellung von menschlichem Insulin aus nichtmenschlichem Insulin-.

Title (fr)

Méthode de préparation d'insuline humaine à partir d'insuline non-humaine-.

Publication

EP 0087238 A1 19830831 (EN)

Application

EP 83300603 A 19830207

Priority

  • GB 8203526 A 19820208
  • GB 8232809 A 19821117

Abstract (en)

Method for the enzymatic replacement of a C-terminal amino acid in an insulin or insulin-like compound with another amino compound or for the addition of an amino compound to a des-insulin or des-insulin-like compound. In the preferred embodiment the method permits the conversion of swine insulin to human insulin in high yield and in short reaction times by preparing a reaction mixture comprising an insulin or insulin-like compound having a first C-terminal amino acid; an amino compound or salt thereof; a solvent system comprising a buffer, capable of maintaining the pH of the reaction mixture between about 5 and 7, and between about 75% and 97% (vol/vol) of at least one non-aqueous reaction mixture miscible solvent, said reaction mixture miscible solvent including at least about 50% (vol/vol) butane-1, 4-diol, and between about 3% and 25% (vol/vol) water; and at least one protease specific to the cleavage of said first C-terminal amino acid from the insulin or insulin-like compound and at least one protease capable joining to that insulin or insulin-like compound said amino compound or salt thereof, but substantially incapable of cleaving the insulin or insulin-like compound elsewhere under the conditions used; and maintaining the temperature of the mixture between about 4 DEG C and 6 DEG C for about 1 to 2 hours.

IPC 1-7

C12P 21/02

IPC 8 full level

C07K 14/62 (2006.01); A61K 38/00 (2006.01)

CPC (source: EP)

C07K 14/62 (2013.01); A61K 38/00 (2013.01)

Citation (search report)

  • [X] EP 0045187 A1 19820203 - FORENEDE BRYGGERIER AS [DK]
  • [X] EP 0017938 A1 19801029 - SHIONOGI & CO [JP]
  • [X] GB 2069502 A 19810826 - NOVO INDUSTRI AS
  • [XP] EP 0056951 A1 19820804 - HOECHST AG [DE]
  • [X] EP 0017485 A1 19801015 - FORENEDE BRYGGERIER AS [DK]
  • [X] US 3276961 A 19661004 - MIKLOS BODANSZKY, et al
  • [X] US 3903068 A 19750902 - RUTTENBERG MICHAEL A
  • [XP] WO 8204069 A1 19821125 - NORDISK INSULINLAB [DK], et al
  • [Y] GB 1426061 A 19760225 - ICI LTD
  • [XP] CHEMICAL ABSTRACTS, vol. 97, no. 13, 1982, page 481, no. 108524r, Columbus, Ohio, USA & JP - A - 82 79898 (SEIKAGAKU KOGYO CO., LTD.) 19-05-1982
  • [X] CHEMICAL ABSTRACTS, vol. 94, no. 13, 30th March 1981, page 794, no. 103820n, Columbus, Ohio, USA
  • [X] BIOLOGICAL ABSTRACTS, vol. 69, no. 10, 1980, page 165, no. 64172, Philadelphia, PA, USA
  • [X] CHEMICAL ABSTRACTS, vol. 96, no. 15, 12th April 1982, page 750, no. 123279n, Columbus, Ohio, USA
  • [X] CHEMICAL ABSTRACTS, vol. 95, no. 3, 20th July 1981, page 759, no. 25640h, Columbus, Ohio, USA & JP - A - 80 138 392 (SHIONOGI AND CO., LTD.) 29-10-1980
  • [X] CHEMICAL ABSTRACTS, vol. 93, no. 25, 22nd December 1980, page 905, no. 239908j, Columbus, Ohio, USA
  • [X] NATURE, vol. 280, 2nd August 1979, pages 412-413, MacMillan Journals Ltd.
  • [X] CHEMICAL ABSTRACTS, vol. 98, no. 5, 31st January 1983, page 683, no. 34928x, Columbus, Ohio, USA
  • [Y] CHEMICAL ABSTRACTS, vol. 96, no. 9, 1st March 1982, page 655, no. 69396h, Columbus, Ohio, USA
  • [Y] CHEMICAL ABSTRACTS, vol. 75, no. 25, 20th December 1971, page 133, no. 149416w, Columbus, Ohio, USA

Designated contracting state (EPC)

AT BE CH DE FR GB IT LI LU NL SE

DOCDB simple family (publication)

EP 0087238 A1 19830831; WO 8302772 A1 19830818

DOCDB simple family (application)

EP 83300603 A 19830207; GB 8300034 W 19830207