EP 0088994 B1 19910619 - HYBRID DNA, BINDING COMPOSITION PREPARED THEREBY AND PROCESSES THEREFOR
Title (en)
HYBRID DNA, BINDING COMPOSITION PREPARED THEREBY AND PROCESSES THEREFOR
Publication
Application
Priority
US 35841482 A 19820315
Abstract (en)
[origin: EP0088994A2] Transformed expression vectors or plasmids carry a ds DNA sequence that codes for only a desired part of a polypeptide chain of a protein or enzyme and is equipped for initiation and termination codons at the 5 min - and 3 min -termini respectively of said DNA sequence; in particular they carry a ds DNA sequence that codes for a variable region of a light or heavy chain of an immunoglobulin specific for a predetermined ligand but lacks nucleotides coding for aminoacid residues superfluous to said variable region. Methods for their preparation and hosts transformed by them are also disclosed. Cultivation of such transformed hosts yields the desired polypeptides, which can be isolated. Preferred polypeptides are provided in the form of compositions containing the variable regions of the light and heavy chains of immunoglobulins, since these are useful especially for diagnosis and therapy.
IPC 1-7
IPC 8 full level
C12N 15/09 (2006.01); A61K 39/395 (2006.01); A61P 37/00 (2006.01); C07H 21/04 (2006.01); C07K 1/22 (2006.01); C07K 14/00 (2006.01); C07K 16/00 (2006.01); C07K 19/00 (2006.01); C12N 1/00 (2006.01); C12N 15/00 (2006.01); C12N 15/10 (2006.01); C12N 15/13 (2006.01); C12P 21/00 (2006.01); C12P 21/02 (2006.01); C12P 21/08 (2006.01); G01N 33/531 (2006.01); G01N 33/577 (2006.01); A61K 38/00 (2006.01); C12R 1/125 (2006.01); C12R 1/19 (2006.01); C12R 1/865 (2006.01)
CPC (source: EP US)
A61P 37/00 (2017.12 - EP); C07K 16/00 (2013.01 - EP US); C12N 15/10 (2013.01 - EP US); G01N 33/531 (2013.01 - EP US); A61K 38/00 (2013.01 - EP US); C07K 2317/622 (2013.01 - EP US); Y10S 424/801 (2013.01 - EP US); Y10S 530/808 (2013.01 - EP US); Y10S 530/809 (2013.01 - EP US); Y10S 530/866 (2013.01 - EP US); Y10S 530/867 (2013.01 - EP US); Y10S 930/30 (2013.01 - EP US)
Designated contracting state (EPC)
AT BE CH DE FR GB IT LI NL SE
DOCDB simple family (publication)
EP 0088994 A2 19830921; EP 0088994 A3 19851009; EP 0088994 B1 19910619; AT E64618 T1 19910715; AU 1241783 A 19830922; AU 560007 B2 19870326; DE 3382317 D1 19910725; DK 115683 A 19830916; DK 115683 D0 19830310; HU 196842 B 19890130; IE 57115 B1 19920422; IE 830537 L 19830915; JP H0645640 B2 19940615; JP S58162599 A 19830927; NZ 203533 A 19860808; US 4642334 A 19870210; US 5840545 A 19981124; US 5851801 A 19981222; ZA 831655 B 19831130
DOCDB simple family (application)
EP 83102288 A 19830309; AT 83102288 T 19830309; AU 1241783 A 19830311; DE 3382317 T 19830309; DK 115683 A 19830310; HU 86083 A 19830314; IE 53783 A 19830311; JP 4053783 A 19830311; NZ 20353383 A 19830310; US 46089295 A 19950605; US 46107195 A 19950605; US 55855183 A 19831205; ZA 831655 A 19830310