EP 0091911 A4 19840406 - GLUCOSE OXIDASE IMMUNOHISTOCHEMICAL DETECTION OF ANTINUCLEAR ANTIBODIES.
Title (en)
GLUCOSE OXIDASE IMMUNOHISTOCHEMICAL DETECTION OF ANTINUCLEAR ANTIBODIES.
Title (de)
IMMUNOHISTOCHEMISCHER NACHWEIS VON ANTINUKLEAREN ANTIKÖRPERN MIT GLUKOSEOXIDASE.
Title (fr)
DETECTION IMMUNOHISTOCHIMIQUE A L'OXYDASE DE GLUCOSE D'ANTICORPS ANTI-NUCLEAIRES.
Publication
Application
Priority
US 29811281 A 19810831
Abstract (en)
[origin: WO8300877A1] For use in the immunohistochemical detection of antigens and related antibodies in a test serum, an antigen source fixed on a support and stabilized by dehydration treatment with an organic solvent mixture, such as aceton methanol, or mixtures thereof. The support is stored under nitrogen in a sealed polyethylene bag. Further, an immunoenzymatic method for the detection of biological components for a test sample, the method comprising the steps of: contacting the stabilized antigen source with the test sample, contacting the antigen source with an antibody conjugated with glucose oxidase and reactive with the component; incubating the antigen source with a solution containing glucose and a chromagenic mixture; and analyzing the antigen source for the presence of color, preferably with a light microscope.
IPC 1-7
IPC 8 full level
G01N 33/552 (2006.01); G01N 33/567 (2006.01)
CPC (source: EP)
G01N 33/552 (2013.01); G01N 33/567 (2013.01)
Citation (search report)
[X] EP 0008720 A1 19800319 - BEHRINGWERKE AG [DE]
Designated contracting state (EPC)
AT BE CH DE FR GB LI NL
DOCDB simple family (publication)
WO 8300877 A1 19830317; CA 1206092 A 19860617; EP 0091911 A1 19831026; EP 0091911 A4 19840406; ES 515350 A0 19831101; ES 524183 A0 19841101; ES 8400773 A1 19831101; ES 8500999 A1 19841101; IT 1153188 B 19870114; IT 8223075 A0 19820831
DOCDB simple family (application)
US 8201107 W 19820812; CA 410409 A 19820830; EP 82902930 A 19820812; ES 515350 A 19820830; ES 524183 A 19830716; IT 2307582 A 19820831