EP 0099255 A3 19850424 - METHOD FOR TRANSFORMING PLANT CELLS
Title (en)
METHOD FOR TRANSFORMING PLANT CELLS
Publication
Application
Priority
US 39761282 A 19820712
Abstract (en)
[origin: EP0099255A2] A method for transforming a plant cell in vitro with a Ti plasmid. The method involves inoculating the plant cell with a strain of Agrobacterium tumefaciens, containing the Ti plasmid, in the presence of an opine metabolite, that is normally synthesized only by a plant cell which has already been transformed by the Ti plasmid, or in the presence of the precursors of the opine metabolite. However, the opine metabolite or its precursors, which are used, must also be able to induce conjugative activity of the Ti plasmid.
IPC 1-7
IPC 8 full level
C12N 15/09 (2006.01); A01H 1/00 (2006.01); C12N 15/82 (2006.01)
CPC (source: EP US)
C12N 15/8205 (2013.01 - EP US)
Citation (search report)
- [A] NATURE, vol. 276, 30th November 1978, pages 498-500, Macmillan Journals Ltd., GB; V.A. SMITH et al.: "Effect of agrocin 84 on attachment of Agrobacterium tumefaciens to cultured tobacco cells"
- [A] NATURE, vol. 277, 11th January 1979, pages 129-131, Macmillan Journals Ltd., GB; L. MARTON et al.: "In vitro transformation of cultured cells from Nicotiana tabacum by Agrobacterium tumefaciens"
- [A] NATURE, vol. 296, 4th March 1982, pages 72-74, Macmillan Journals Ltd., GB; F.A. KRENS et al.: "In vitro transformation of plant protoplasts with Ti-plasmid DNA"
- NATURE, vol. 296, 4th March 1982, pages 72-74, Macmillan Journals Ltd., GB; F.A. KRENS et al.: "In vitro transformation of plant protoplasts with Ti-plasmid DNA"
Designated contracting state (EPC)
BE CH DE FR GB LI NL
DOCDB simple family (publication)
EP 0099255 A2 19840125; EP 0099255 A3 19850424; CA 1195628 A 19851022; JP S5963187 A 19840410; US 4459355 A 19840710
DOCDB simple family (application)
EP 83303990 A 19830708; CA 431396 A 19830629; JP 12683383 A 19830712; US 39761282 A 19820712