EP 0105359 A4 19840820 - METHOD OF AFFINITY PURIFICATION EMPLOYING MONOCLONAL ANTIBODIES.
Title (en)
METHOD OF AFFINITY PURIFICATION EMPLOYING MONOCLONAL ANTIBODIES.
Title (de)
VERFAHREN ZUR AFFINITÄTSREINIGUNG MITTELS MONOKLONALER ANTIKÖRPER.
Title (fr)
PROCEDE DE PURIFICATION PAR AFFINITE UTILISANT DES ANTICORPS MONOCLONAUX.
Publication
Application
Priority
US 36778182 A 19820412
Abstract (en)
[origin: WO8303678A1] Process for affinity purification of antigens and antibodies that employs monoclonal antibodies having a high affinity for the antigen in a first environment and a low affinity in a second environment, the environments being ones in which the immuno-chemical properties of the antigens and antibodies are not adversely affected. Also described is a process for fractionating antisera to obtain an antibody fraction having similar antigen binding properties as exhibited by the environmentally sensitive monoclonal antibodies. The effect of varying pH in the binding capability of tow monoclonal antibodies for human growth hormone is shown in fig. 1.
IPC 1-7
IPC 8 full level
C07K 16/18 (2006.01); C07K 16/46 (2006.01); C12N 9/16 (2006.01); C12P 21/08 (2006.01)
CPC (source: EP)
C07K 16/18 (2013.01); C07K 16/468 (2013.01); C12N 9/16 (2013.01)
Citation (search report)
- [XP] EP 0068763 A2 19830105 - UNIV TEXAS [US]
- [XP] US 4361509 A 19821130 - ZIMMERMAN THEODORE S, et al
- [XP] WO 8202818 A1 19820902 - UNILEVER PLC [GB], et al
Designated contracting state (EPC)
BE DE FR NL SE
DOCDB simple family (publication)
WO 8303678 A1 19831027; AT 393386 B 19911010; AT A901983 A 19910315; AU 622099 B2 19920402; AU 7256987 A 19870903; CA 1209907 A 19860819; CH 672028 A5 19891013; DK 192191 A 19911126; DK 192191 D0 19911126; EP 0105359 A1 19840418; EP 0105359 A4 19840820; ES 521371 A0 19840516; ES 528000 A0 19850801; ES 528001 A0 19870701; ES 8404858 A1 19840516; ES 8506902 A1 19850801; ES 8706964 A1 19870701; FI 834530 A0 19831209; FI 834530 A 19831209; FI 88403 B 19930129; FI 88403 C 19930510; GB 2128630 A 19840502; GB 2128630 B 19860828; GB 8332644 D0 19840111; IT 1219779 B 19900524; IT 8320550 A0 19830412
DOCDB simple family (application)
US 8300524 W 19830412; AT 901983 A 19830412; AU 7256987 A 19870506; CA 425555 A 19830411; CH 669983 A 19830412; DK 192191 A 19911126; EP 83901671 A 19830412; ES 521371 A 19830411; ES 528000 A 19831213; ES 528001 A 19831213; FI 834530 A 19831209; GB 8332644 A 19830412; IT 2055083 A 19830412