Title (en)

PLASMINOGEN ACTIVATOR

Publication

EP 0112122 A3 19870401 (EN)

Application

EP 83307468 A 19831208

Priority

• AU 1029683 A 19830111
• ZA 829168 A 19821214

Abstract (en)

[origin: EP0112122A2] The invention provides a process for producing a purified plasminogen activator substance based on the TPA/pro-TPA couple. Pro-TPA yielding cell cultures are cultivated in a suitable culture medium in the absence of enzymes which catalyse the conversion of pro-TPA into TPA or where such enzymes are present in the presence of a suitable inhibitor adapted to inhibit such enzymes. Accordingly the couple is represented in the harvesting liquid by at least 50 to 100% of pro-TPA and can in fact be virtually 100% pro-TPA, any balance being TPA. The harvesting fluid is treated with an affinity reagent comprising an immobilised Kunitz type inhibitor of the type occurring in seeds of Erythrina latissima and other Erythrina species (DE-3) to selectively adsorb the pro-TPA and any TPA in the harvesting fluid, followed by desorption of pro-TPA and optionally TPA. The pro-TPA so recoverable has 1/10 the plasminogen activating properties of TPA. It is convertable into active TPA enzymatically, e.g. by plasmin or by enzymes present in fibrin clots. The pro-TPA has a higher affinity for fibrin and better selectivity than TPA. It is used for the management and prophylaxis of thrombosis and embolism in dosages of pro-TPA/TPA couple which result in less than 50%, e.g. less than 20% of the plasminogen activating activity resulting from up to 15 mg/24 hrs pure TPA.

IPC 1-7

C12N 9/64; A61K 37/54

IPC 8 full level

C12N 9/72 (2006.01); A61K 38/00 (2006.01)

CPC (source: EP US)

C12N 9/6459 (2013.01 - EP US); C12Y 304/21069 (2013.01 - EP US); A61K 38/00 (2013.01 - EP US); Y10S 435/814 (2013.01 - EP US); Y10S 435/815 (2013.01 - EP US)

Citation (search report)

• EP 0041766 A2 19811216 - LEUVEN RES & DEV VZW [BE]
• EP 0023860 A2 19810211 - FABRE SA PIERRE [FR]
• EP 0113319 A2 19840711 - WILSON ELAINE LYNETTE
• US 4245051 A 19810113 - REICH EDWARD, et al
• GB 2103791 A 19830223 - SOUTH AFRICAN INVENTIONS [ZA]

Designated contracting state (EPC)

CH DE FR GB LI SE

DOCDB simple family (publication)

EP 0112122 A2 19840627; EP 0112122 A3 19870401; EP 0112122 B1 19910828; DE 3382389 D1 19911002; US 4902623 A 19900220

DOCDB simple family (application)

EP 83307468 A 19831208; DE 3382389 T 19831208; US 91559986 A 19861003