EP 0125867 A3 19870107 - ASSAY SYSTEMS USING MORE THAN ONE ENZYME

Title (en)

ASSAY SYSTEMS USING MORE THAN ONE ENZYME

Publication

EP 0125867 A3 19870107 (EN)

Application

EP 84303085 A 19840508

Priority

GB 8312259 A 19830505
GB 8312265 A 19830505
GB 8323800 A 19830906
GB 8323801 A 19830906
GB 8405262 A 19840229
GB 8405263 A 19840229

Abstract (en)

[origin: EP0125867A2] This specification discloses methods of detection or measurement of an enzyme or of its specific substrate, and sensors used in such methods. The present invention is con- cenred with a multi-enzyme system, and the specification discloses, as one aspect of the invention a method of assay in which an electrode poised at a suitable potential is contacted with a system comprising a first enzyme, a cofactor linked with said enzyme and a mediator compound which transfers charge to the electrode from the first enzyme when its electrical state is changed by reaction of cofactor material.The cofactor may be NAD, NADP (both collectively referred to herein as NAD (P) CAMP ATP GTP TTP CTP.The specification particularly illustrates a method of assay in which an electrode (1) poised at a suitable potential is contact with a system comprising a first enzyme El a nicotinamide adenine dinucleotide compound N linked with said enzyme EI and a mediator compound F which transfers charge to the electrode from the first enzyme when its electrical state is changed by a NAD(P) NAD(P)H reaction. The NAD compound may act as a "bridge" between the said enryme// mediator system and further NAD utilizing enzymes E2.

IPC 1-7

C12Q 1/00; C12Q 1/32; C12Q 1/54

IPC 8 full level

A61B 5/00 (2006.01); C12Q 1/00 (2006.01); C12Q 1/68 (2006.01)

CPC (source: EP US)

A61B 5/1486 (2013.01 - EP US); A61B 5/6848 (2013.01 - EP US); C12Q 1/001 (2013.01 - EP US); C12Q 1/004 (2013.01 - EP US); Y10S 435/817 (2013.01 - EP US)

Citation (search report)

[YP] EP 0078636 A1 19830511 - GENETICS INT INC [US], et al
[YP] US 4392933 A 19830712 - NAKAMURA KENICHI [JP], et al
[A] EP 0007058 A1 19800123 - BOEHRINGER MANNHEIM GMBH [DE]
[Y] WO 8000453 A1 19800320 - NAT RES DEV [GB], et al
[A] ANALYTICAL CHEMISTRY, vol. 54, no. 12, October 1982, pages 1987-1990, American Chemical Society, Easton, Pennsylvania, US; H. WINARTASAPUTRA et al.: "Amperometric enzymic determination of triglycerides in serum"

Designated contracting state (EPC)

BE CH DE FR GB IT LI NL SE

DOCDB simple family (publication)

EP 0125867 A2 19841121; EP 0125867 A3 19870107; CA 1218704 A 19870303; US 4758323 A 19880719

DOCDB simple family (application)

EP 84303085 A 19840508; CA 453583 A 19840504; US 60769884 A 19840507