EP 0130756 A1 19850109 - Procaryotic carbonyl hydrolases, methods, DNA, vectors and transformed hosts for producing them, and detergent compositions containing them.
Title (en)
Procaryotic carbonyl hydrolases, methods, DNA, vectors and transformed hosts for producing them, and detergent compositions containing them.
Title (de)
Prokaryotische Carbonyl-Hydrolasen, Verfahren, DNA, Vektoren und transformierte Wirte zu ihrer Herstellung und diese Hydrolasen enthaltende Detergenszusammensetzungen.
Title (fr)
Carbonyl-hydrolases procaryotiques, méthodes, ADN, vecteurs et hÔtes transformés pour leur production, et compositions des détergents contenant les dites hydrolases.
Publication
Application
Priority
- US 50741983 A 19830624
- US 61449184 A 19840529
- US 61461284 A 19840529
- US 61461584 A 19840529
- US 61461684 A 19840529
- US 61461784 A 19840529
Abstract (en)
Methods and vectors are provided for the production of procaryotic carbonyl hydrolases in recombinant systems. DNA which encodes such hydrolases are mutated at predetermined regions by known methods or by a novel point mutagenesis method in order to generate mutant hydrolases. Particular point mutations in carbonyl hydrolases such as subtilisin result in modifications of oxidation stability, Km, Kcat, Kcat/Km ratio substrate specificity, specific activity or pH-activity profiles. These mutated hydrolases are particularly useful in laundry compositions. Mutations in the genes encoding the subtilisin or neutral protease of bacillus yield substantially normally sporulating bacillus strains which are incapable of excreting subtilisin or neutral protease. Such strains are useful in the recombinant synthesis of heterologous proteins.
IPC 1-7
C11D 3/386; C11D 7/42; C12N 9/14; C12N 9/54; C12N 15/00; C12N 15/57; C12R 1/125
IPC 8 full level
C07H 21/04 (2006.01); C11D 3/386 (2006.01); C11D 7/42 (2006.01); C12N 1/20 (2006.01); C12N 1/21 (2006.01); C12N 9/14 (2006.01); C12N 9/26 (2006.01); C12N 9/54 (2006.01); C12N 9/56 (2006.01); C12N 9/58 (2006.01); C12N 15/00 (2006.01); C12N 15/09 (2006.01); C12N 15/10 (2006.01); C12N 15/57 (2006.01); C12N 15/75 (2006.01); C12R 1/07 (2006.01); C12R 1/125 (2006.01)
CPC (source: EP)
C11D 3/386 (2013.01); C12N 1/205 (2021.05); C12N 9/54 (2013.01); C12N 15/102 (2013.01); C12N 15/75 (2013.01); C12R 2001/07 (2021.05)
Citation (search report)
- EP 0092182 A2 19831026 - GENENTECH INC [US]
- EP 0057976 A2 19820818 - CPC INTERNATIONAL INC [US]
- US 3790482 A 19740205 - JONES J, et al
- US 3557002 A 19710119 - MCCARTY CHARLES BRUCE
- [AD] NATURE, vol. 299, no. 5885, October 21, 1982, New York, London G. WINTER et al. "Redesigning enzyme structure by sitedirected mutagenesis: tyrosyl t RNA synthetase and ATP binding" pages 756-758
- [APD] NATURE, vol. 307, January 12, 1984, New York, London A.J. WILKINSON et al. "A large increase in enzym-substrate affinity by protein engineering" pages 187-188
Designated contracting state (EPC)
AT BE CH DE FR GB IT LI LU NL SE
DOCDB simple family (publication)
EP 0357157 A2 19900307; EP 0357157 A3 19900328; AT E60356 T1 19910215; AT E60797 T1 19910215; DE 3484019 D1 19910228; DE 3484079 D1 19910314; DE 3486139 D1 19930603; DE 3486139 T2 19931111; DK 175278 B1 20040802; DK 175768 B1 20050214; DK 305984 A 19850218; DK 305984 D0 19840622; DK 82293 A 19930708; DK 82293 D0 19930708; DK 82393 A 19930708; DK 82393 D0 19930708; EP 0130756 A1 19850109; EP 0130756 B1 19910206; EP 0130756 B2 20000628; EP 0246678 A1 19871125; EP 0246678 B1 19930428; EP 0247647 A1 19871202; EP 0247647 B1 19910123; HK 75393 A 19930806; HK 75493 A 19930806; HK 99293 A 19930930; IE 81141 B1 20000405; IE 841567 L 19841224; JP 2594533 B2 19970326; JP 2889095 B2 19990510; JP H06315378 A 19941115; JP H06319534 A 19941122; JP S6070075 A 19850420; NZ 208612 A 19910925
DOCDB simple family (application)
EP 89202584 A 19840622; AT 84304252 T 19840622; AT 87200689 T 19840622; DE 3484019 T 19840622; DE 3484079 T 19840622; DE 3486139 T 19840622; DK 305984 A 19840622; DK 82293 A 19930708; DK 82393 A 19930708; EP 84304252 A 19840622; EP 87200689 A 19840622; EP 87200690 A 19840622; HK 75393 A 19930729; HK 75493 A 19930729; HK 99293 A 19930923; IE 156784 A 19840621; JP 12992884 A 19840622; JP 24482393 A 19930930; JP 24483793 A 19930930; NZ 20861284 A 19840621