EP 0133282 B1 19911023 - PREPARATION OF ACIDAMIDE-CARBOXY-TERMINAL POLYPEPTIDES
Title (en)
PREPARATION OF ACIDAMIDE-CARBOXY-TERMINAL POLYPEPTIDES
Publication
Application
Priority
DE 3327007 A 19830727
Abstract (en)
[origin: EP0133282A1] Polypeptides with a carboxamide carboxyl terminus and a methionine residue, which is optionally bound to a bacterial protein, at the amino end can be prepared by genetic engineering synthesis of the corresponding polypeptide with a glycine residue at the C terminus and the enzymatic conversion thereof into the amino group of the required carboxamide moiety. Peptides which have the amino-acid sequence of growth hormone releasing factor, part sequences thereof or modifications of these peptides can easily be obtained in this way. The genetic engineering synthesis is expediently carried out using two gene fragments which are chemically synthesised from single-stranded smaller fragments. The two gene fragments are then linked enzymatically to give the complete gene, incorporated in a suitable vector, amplified there, the peptide is isolated directly or as fusion protein and is converted enzymatically into the required amide.
IPC 1-7
C07K 1/00; C07K 7/08; C07K 7/10; C07K 15/04; C12N 15/31; C12P 21/00; C12P 21/02
IPC 8 full level
C12N 15/09 (2006.01); C07H 21/00 (2006.01); C07H 21/04 (2006.01); C07K 1/00 (2006.01); C07K 7/08 (2006.01); C07K 14/00 (2006.01); C07K 14/575 (2006.01); C07K 14/60 (2006.01); C07K 19/00 (2006.01); C12N 1/20 (2006.01); C12N 15/00 (2006.01); C12N 15/16 (2006.01); C12N 15/31 (2006.01); C12P 21/00 (2006.01); C12P 21/02 (2006.01); C12R 1/19 (2006.01)
CPC (source: EP KR US)
C07H 21/00 (2013.01 - EP US); C07K 14/60 (2013.01 - EP US); C12N 15/00 (2013.01 - KR); C12P 21/02 (2013.01 - EP US); C07K 2319/00 (2013.01 - EP US); C07K 2319/75 (2013.01 - EP US)
Designated contracting state (EPC)
AT BE CH DE FR GB IT LI LU NL SE
DOCDB simple family (publication)
EP 0133282 A1 19850220; EP 0133282 B1 19911023; AT E68827 T1 19911115; AU 3121784 A 19850131; AU 572171 B2 19880505; CA 1224166 A 19870714; DE 3327007 A1 19850207; DE 3485199 D1 19911128; DK 171940 B1 19970818; DK 365484 A 19850128; DK 365484 D0 19840726; ES 534589 A0 19851016; ES 8601303 A1 19851016; GR 82436 B 19841213; IE 57664 B1 19930224; IE 841930 L 19850127; IL 72489 A0 19841130; JP H07121229 B2 19951225; JP S6049798 A 19850319; KR 850001287 A 19850318; PT 78979 A 19840801; US 5457033 A 19951010; ZA 845779 B 19850227
DOCDB simple family (application)
EP 84108766 A 19840725; AT 84108766 T 19840725; AU 3121784 A 19840726; CA 459771 A 19840726; DE 3327007 A 19830727; DE 3485199 T 19840725; DK 365484 A 19840726; ES 534589 A 19840724; GR 840175419 A 19840725; IE 193084 A 19840726; IL 7248984 A 19840725; JP 15418884 A 19840726; KR 840004448 A 19840726; PT 7897984 A 19840726; US 20581694 A 19940304; ZA 845779 A 19840726