EP 0134078 A3 19860312 - IMPROVED METHOD OF DETERMINING ANTIBIOTICS IN BIOLOGICAL LIQUIDS
Title (en)
IMPROVED METHOD OF DETERMINING ANTIBIOTICS IN BIOLOGICAL LIQUIDS
Publication
Application
Priority
US 52185783 A 19830810
Abstract (en)
[origin: EP0134078A2] The presence of beta lactum ring-containing cephalosporins and penicillin is determined in biological liquids such as milk by contacting the test liquid with a beta lactum ring-containing chromogenic compound and penicillinase and measuring after a predetermined time period the colour developed in the test liquid in comparison with a colour standard. Preferably, the colour standard is provided by contacting the same ingredients and concentrations for the same time period, exept that the liquid is free of beta lactum ring-containing cephalosporin and penicillin. The colourimetric test can be carried out within a few minutes (e.g. 3-15) utilizing a chromegenic compound such as nitrocefin, preferably on a solid phase substrate. The penicillinase attacks both the beta lactum ring of the chromogenic compound and any beta lactum ring-containing cephalosporin and/or penicillin present in the test liquid. The generation of colour due to breakage of the chromogenic compound's beta lactum ring is correspondingly reduced as the concentration of the beta lactum ring-containing antibiotic increases in the test liquid. Therefore, the method permits measurement of the concentration of such antibiotic in the test liquid. The method is simple, inexpensive, rapid and effective and can be run on equipment which is readily available in most laboratories.
IPC 1-7
IPC 8 full level
C12Q 1/34 (2006.01); G01N 33/04 (2006.01)
CPC (source: EP US)
C12Q 1/34 (2013.01 - EP US); G01N 33/04 (2013.01 - EP US); G01N 33/9446 (2013.01 - EP US); G01N 2333/986 (2013.01 - EP US); G01N 2415/00 (2013.01 - EP US)
Citation (search report)
- [A] US 2601350 A 19520624 - HENRY WELCH
- [A] EP 0037932 A1 19811021 - TEVA PHARMA [IL], et al
- [A] EP 0059645 A1 19820908 - NAT RES DEV [GB]
- [E] EP 0118274 A1 19840912 - UNILEVER PLC [GB], et al
- [A] EP 0005891 A1 19791212 - GIST BROCADES NV [NL]
- [A] CLINICAL CHEMISTRY, vol. 27, no. 9, September 1981, pages 1490-1498, Easton, PA., US; R.H. YOLKEN "Enzymic analysis for rapid detection of microbial infection in human body fluids: an overview"
Designated contracting state (EPC)
DE FR IT NL
DOCDB simple family (publication)
EP 0134078 A2 19850313; EP 0134078 A3 19860312; AU 2764884 A 19850214; AU 558345 B2 19870129; US 4568637 A 19860204
DOCDB simple family (application)
EP 84303966 A 19840612; AU 2764884 A 19840503; US 52185783 A 19830810