Global Patent Index - EP 0144913 A3

EP 0144913 A3 19860820 - HYBRIDIZATION ASSAY EMPLOYING LABELED PROBE AND ANTI-HYBRID

Title (en)

HYBRIDIZATION ASSAY EMPLOYING LABELED PROBE AND ANTI-HYBRID

Publication

EP 0144913 A3 19860820 (EN)

Application

EP 84114442 A 19841129

Priority

  • US 56042983 A 19831212
  • US 64585084 A 19840831
  • US 66825584 A 19841107

Abstract (en)

[origin: EP0144913A2] A nucleic acid hybridization assay involving a labeled probe and formation of a hybrid having epitopes for an antibody reagent. The label provides a detectable response which is measurably different when the labeled probe is comprised in a hybrid that is bound by the antibody reagent compared to when not comprised in such a hybrid. Particularly useful antibody reagents are antibodies such as anti-DNA<.>RNA, anti-RNA<.>RNA and antibodies to intercalated duplexes which do not bind substantially to single stranded nucleic acids. Modulation of the label response can be accomplished in a variety of ways such as by steric inactivation or hindrance or by labeling the antibody reagent with a second label which interacts with the first label. The assay is particularly advantageous because no separation of hybridized and unhybridized probe is required.

IPC 1-7

C12Q 1/68; G01N 33/542

IPC 8 full level

C07K 16/18 (2006.01); C12Q 1/68 (2006.01); C12Q 1/70 (2006.01)

CPC (source: EP)

C07K 16/18 (2013.01); C12Q 1/6804 (2013.01); C12Q 1/6816 (2013.01); C12Q 1/6818 (2013.01)

Citation (search report)

  • [E] EP 0133671 A2 19850306 - MILES LAB [US]
  • [Y] EP 0087564 A1 19830907 - MILES LAB [US]
  • [Y] EP 0095089 A1 19831130 - MILES LAB [US]
  • [Y] EP 0093613 A1 19831109 - SYVA CO [US]
  • [AD] EP 0070685 A2 19830126 - STANDARD OIL CO [US]
  • [YD] EP 0063879 A2 19821103 - UNIV YALE [US]
  • [Y] BIOCHEMISTRY, vol. 17, no. 26, 26th December 1978, pages 5791-5798, American Chemical Society, US; W.E. STUMPH et al.: "Gene enrichment using antibodies to DNA/RNA hybrids: Purification and mapping of Dictyostelium discoideum rDNA"
  • [A] PROC. NATL. ACAD. SCI., USA, vol. 79, no. 15, August 1982, pages 4594-4598; W.A. SAFFRAN et al.: "Site-directed psoralen crosslinking of DNA"
  • [A] BIOCHEMISTRY, vol. 18, no. 7, 4th April 1979, pages 1328-1332, American Chemical Society, US; E. SAGE et al.: "Reactivity of the antibodies to DNA modified by the carcinogen N-acetoxy-N-acetyl-2-aminofluorene"
  • [Y] CHEMICAL ABSTRACTS, vol. 70, no. 25, 23rd June 1969, page 168, no. 113344d, Columbus, Ohio, US; E.F. SCHWARTZ et al.: "Antibodies to polyadenylate-polyuridylate copolymers as reagents for double-strand RNA and DNA-RNA hybrid complexes", & BIOCHEM. BIOPHYS. RES. COMMUN. 1969, 35(1), 115-20

Designated contracting state (EPC)

AT BE CH DE FR GB IT LI LU NL SE

DOCDB simple family (publication)

EP 0144913 A2 19850619; EP 0144913 A3 19860820; DK 591684 A 19850613; DK 591684 D0 19841211; ES 538543 A0 19860516; ES 8607559 A1 19860516; FI 844869 A0 19841210; FI 844869 L 19850613

DOCDB simple family (application)

EP 84114442 A 19841129; DK 591684 A 19841211; ES 538543 A 19841212; FI 844869 A 19841210