EP 0179025 B1 19920729 - METHOD FOR THE PRODUCTION OF NEUTRAL PROTEASE
Title (en)
METHOD FOR THE PRODUCTION OF NEUTRAL PROTEASE
Publication
Application
Priority
IT 2319084 A 19841017
Abstract (en)
[origin: EP0179025A1] Method for the production of neutral protease comprising: a) constructing a hybrid recombinant plasmid containing the gene which codes for the neutral protease, b) isolating a mutant strain of Bacillus subtilis, c) introducing the recombinant hybrid plasmid into the mutant strain, d) isolating the clones containing the hybrid plasmid, e) cultivating the clones in a liquid culture medium, under areobic conditions, f) isolating the neutral protease obtained from the culture medium.
IPC 1-7
IPC 8 full level
C12N 9/56 (2006.01); C12N 15/00 (2006.01); C12N 15/57 (2006.01); C12N 15/09 (2006.01); C12N 15/75 (2006.01); C12R 1/125 (2006.01)
CPC (source: EP)
C12N 9/54 (2013.01); C12N 15/75 (2013.01)
Citation (examination)
- J. Bacteriol. 154 (1983), 831-837;
- J. Bacteriol. 160 (1984) 15-21;
- J. Bacteriol. 159 (1984) 811-819
- Mol. Gen. Genet. 181 (1981) 434-440
- J. Bacteriol. 139 (1979) 775-782
- J. Bacteriol. 119 (1974) 82-91
- Bacillus Genetic Stock Center Catalog of Strains, 2nd edition, page 32
Designated contracting state (EPC)
AT BE CH DE FR GB LI LU NL SE
DOCDB simple family (publication)
EP 0179025 A1 19860423; EP 0179025 B1 19920729; AT E78870 T1 19920815; CA 1285233 C 19910625; DE 3586410 D1 19920903; DE 3586410 T2 19930304; IT 1176997 B 19870826; IT 8423190 A0 19841017; IT 8423190 A1 19860417; JP H0829083 B2 19960327; JP S61181374 A 19860814
DOCDB simple family (application)
EP 85830246 A 19850930; AT 85830246 T 19850930; CA 492088 A 19851002; DE 3586410 T 19850930; IT 2319084 A 19841017; JP 23088785 A 19851016