EP 0184417 A2 19860611 - Apparatus and method for cryopreparing biological tissue for ultrastructural analysis.
Title (en)
Apparatus and method for cryopreparing biological tissue for ultrastructural analysis.
Title (de)
Gerät und Verfahren zur Kryovorbereitung von biologischem Gewebe für die Ultrastrukturanalyse.
Title (fr)
Appareil et méthode pour la cryopréparation de tissu biologique pour l'analyse ultrastructurale.
Publication
Application
Priority
US 67685584 A 19841130
Abstract (en)
This invention relates to apparatus for the cryopreparation of biological tissue samples for ultrastructural analysis. The use of the apparatus comprises vitrifying a biological tissue sample under cryogenic temperature conditions and ultra low vacuum conditions. The depressurized, vitrified tissue sample is brought to equilibrium in a sample holder at a temperature of less than - 140 DEG C. The tissue sample is then dehydrated while maintained in a state of thermal equilibrium. After reaching equilibrium the tissue sample is optionally infiltrated with a degassed resin followed by a polymerization of the resin to form an embedded tissue sample.
IPC 1-7
IPC 8 full level
G01N 1/42 (2006.01); G01N 33/48 (2006.01)
CPC (source: EP US)
G01N 1/42 (2013.01 - EP US)
Designated contracting state (EPC)
AT BE CH DE FR GB IT LI LU NL SE
DOCDB simple family (publication)
US 4567847 A 19860204; AU 4996385 A 19860605; AU 588552 B2 19890921; CA 1239290 A 19880719; CA 1301465 C 19920526; EP 0184417 A2 19860611; EP 0184417 A3 19870422; JP S61198037 A 19860902
DOCDB simple family (application)
US 67685584 A 19841130; AU 4996385 A 19851115; CA 495267 A 19851113; CA 547937 A 19870925; EP 85308760 A 19851202; JP 27058085 A 19851130