EP 0188555 A4 19880418 - YEAST CLONING VEHICLE.
Title (en)
YEAST CLONING VEHICLE.
Title (de)
KLONIERUNGSVEKTOR FÜR HEFE.
Title (fr)
VEHICULE DE CLONAGE DE LEVURE.
Publication
Application
Priority
US 62920384 A 19840709
Abstract (en)
[origin: WO8600637A1] A cloning vehicle with yeast transcription control sequence and a yeast signal sequence placed in correct reading frame and transcripton direction with respect to an exogenous DNA sequence coding for a desired protein. In a particular aspect, the transcription and signal sequences are substantially identical to those of the naturally occurring PHO5 gene, and the signal sequence codes for a signal peptide that has the same three carboxy terminal amino acids as the PHO5 signal peptide. The vehicle is used to transform a yeast host which secretes the desired protein. The vehicle can be made by ligating the exogenous DNA to a DNA fragment having a restriction endonuclease cleavage site that has been inserted at the three 3' end of the signal sequence, with no extraneous base pairs therebetween; alternatively, where G is permitted as the 5' end base pair of the exogenous DNA, the vehicle can be made by ligating the exogenous DNA, minus the base at its 5' end to a DNA fragment where a single G base has been positioned between the 3' end of the signal sequence and the exogenous DNA sequence.
IPC 1-7
IPC 8 full level
C07K 14/59 (2006.01); C12N 15/00 (2006.01); C12N 15/09 (2006.01); C12N 15/62 (2006.01); C12N 15/81 (2006.01); C12P 21/00 (2006.01); C12R 1/865 (2006.01)
CPC (source: EP)
C07K 14/59 (2013.01); C12N 15/625 (2013.01); C12N 15/81 (2013.01); C07K 2319/02 (2013.01); C07K 2319/036 (2013.01); C07K 2319/75 (2013.01)
Citation (search report)
- [XP] EP 0143081 A2 19850529 - CIBA GEIGY AG [CH]
- [XP] EP 0116201 A1 19840822 - CHIRON CORP [US]
- See references of WO 8600637A1
Designated contracting state (EPC)
AT BE CH DE FR GB IT LI LU NL SE
DOCDB simple family (publication)
WO 8600637 A1 19860130; DK 103886 A 19860507; DK 103886 D0 19860307; EP 0188555 A1 19860730; EP 0188555 A4 19880418; JP S61502655 A 19861120
DOCDB simple family (application)
US 8501306 W 19850709; DK 103886 A 19860307; EP 85903625 A 19850709; JP 50324385 A 19850709