EP 0191846 A4 19880427 - METHOD OF PRODUCING MONOCLONAL ANTI-IDIOTYPIC ANTIBODIES.
Title (en)
METHOD OF PRODUCING MONOCLONAL ANTI-IDIOTYPIC ANTIBODIES.
Title (de)
HERSTELLUNG MONOKLONALER ANTI-IDIOTYP-ANTIKÖRPER.
Title (fr)
PROCEDE DE PRODUCTION D'ANTICORPS MONOCLONAUX ANTI-IDIOTYPIQUES.
Publication
Application
Priority
US 64455084 A 19840827
Abstract (en)
[origin: WO8601539A1] An auto-anti-idiotypic method has been developed for producing an monoclonal anti-idiotypic antibody which involves: (a) contacting lymphoid cells of an animal under suitable conditions with an effective antibody-raising amount of an antigen, e.g. a ligand to a receptor; (b) collecting the lymphoid cells from the animal at a suitable time after the contacting; (c) fusing the collected lymphoid cells with appropriate myeloma cells to produce a series of hybridoma cells each of which produces a monoclonal antibody; (d) screening under suitable conditions the series of hybridoma cells so produced to identify those which secrete a monoclonal antibody capable of binding to an antibody directed to the antigen, or to a receptor where the antigen is a ligand to the receptor; (e) separately culturing a hybridoma cell so identified in an appropriate medium, and (f) separately recovering under suitable conditions the monoclonal anti-idiotypic antibody produced by the hybridoma cell. The antigen may be conjugated to a protein, and may be a ligand to a receptor, in which case the monoclonal anti-idiotypic antibody will be capable of binding to the receptor, and preferably acts as an agonist or antagonist to the receptor. By this method a monoclonal anti-idiotypic antibody may be produced and recovered which in a preferred embodiment is capable of binding to a receptor, e.g. a receptor to acetylcholine or adenosine.
IPC 1-7
IPC 8 full level
G01N 33/566 (2006.01); A61K 39/00 (2006.01); C07K 14/005 (2006.01); C07K 14/195 (2006.01); C07K 14/705 (2006.01); C07K 14/76 (2006.01); C07K 16/00 (2006.01); C07K 16/42 (2006.01); C12N 5/00 (2006.01); C12N 5/10 (2006.01); C12N 15/00 (2006.01); C12N 15/02 (2006.01); C12P 21/00 (2006.01); C12P 21/08 (2006.01); G01N 33/577 (2006.01); C12R 1/91 (2006.01)
CPC (source: EP)
C07K 16/4241 (2013.01)
Citation (search report)
- [XPO] CHEMICAL ABSTRACTS, vol. 104, no. 3, 20th January 1986, page 376, abstract no. 18339t, Columbus, Ohio, US; B.F. ERLANGER et al.: "Anti-idiotypic antibody to the acetylcholine and adenosine receptors", & MOL. BASIS NERVE ACT., PROC. INT. SYMP. MEM. DAVID NACHMANSOHN 1984 (PUB. 1985), 523-36
- See references of WO 8601539A1
Designated contracting state (EPC)
AT BE CH DE FR GB IT LI LU NL SE
DOCDB simple family (publication)
WO 8601539 A1 19860313; AU 4777385 A 19860324; EP 0191846 A1 19860827; EP 0191846 A4 19880427; JP S62500421 A 19870226
DOCDB simple family (application)
US 8501658 W 19850827; AU 4777385 A 19850827; EP 85904390 A 19850827; JP 50391785 A 19850827