EP 0200113 A3 19870318 - A METHOD OF SOLID PHASE NUCLEIC ACID HYBRIDIZATION ASSAY INCORPORATING A LUMINESCENT LABEL
Title (en)
A METHOD OF SOLID PHASE NUCLEIC ACID HYBRIDIZATION ASSAY INCORPORATING A LUMINESCENT LABEL
Publication
Application
Priority
US 72900185 A 19850430
Abstract (en)
[origin: EP0200113A2] A method of solid phase nucleic acid hybridization assay which incorporates a first fragment of nucleic acid bound to a solid carrier comprising water insoluble particles and a second fragment of nucleic acid bound to a luminescent label. Hybridization occurs while the solid carrier is suspended and the luminescence is measured after the solid carrier is concentrated by microfiltration.
IPC 1-7
IPC 8 full level
G01N 33/50 (2006.01); C07H 21/00 (2006.01); C12Q 1/68 (2006.01); G01N 21/76 (2006.01); G01N 21/78 (2006.01); G01N 33/543 (2006.01)
CPC (source: EP)
C12Q 1/6813 (2013.01); C12Q 1/6823 (2013.01); G01N 33/54313 (2013.01)
Citation (search report)
- [A] EP 0070687 B1 19851016
- [A] EP 0079139 A1 19830518 - ORION YHTYMAE OY [FI]
- [A] EP 0124124 A2 19841107 - ENZO BIOCHEM INC [US]
- [A] US 3853987 A 19741210 - DREYER W
- [XP] CLINICAL CHEMISTRY, vol. 31, no. 9, September 1985, pages 1438-1443, Winston-Salem, NC, US; R. POLSKY-CYNKIN et al.: "Use of DNA immobilized on plastic and agarose supports to detect DNA by sandwich hybridization"
- [AD] GENE, vol. 21, 1983, pages 77-85, NL; M. RANKI et al.: "Sandwich hybridization as a convenient method for the detection of nucleic acids in crude samples"
Designated contracting state (EPC)
AT BE CH DE FR GB IT LI LU NL SE
DOCDB simple family (publication)
EP 0200113 A2 19861105; EP 0200113 A3 19870318; JP S61264240 A 19861122
DOCDB simple family (application)
EP 86105375 A 19860418; JP 10077786 A 19860430