EP 0221955 A4 19881222 - HIGH LEVEL AMPLIFICATION AND EXPRESSION OF EXOGENOUS DNA.
Title (en)
HIGH LEVEL AMPLIFICATION AND EXPRESSION OF EXOGENOUS DNA.
Title (de)
HOCHSTUFIGE VERSTÄRKUNG UND EXPRESSION VON EXOGENER DNS.
Title (fr)
AMPLIFICATION ET EXPRESSION A UN NIVEAU ELEVE D'ADN EXOGENE.
Publication
Application
Priority
US 72930685 A 19850501
Abstract (en)
[origin: WO8606409A1] A method for producing high level expression of a selected protein and cell line and vectores useful therein. This method involves incorporating an exogenous ADA gene and an exogenous gene coding for a desired protein into a cell line containing an endogenous ADA gene.
IPC 1-7
IPC 8 full level
C12N 15/00 (2006.01); C12N 5/00 (2006.01); C12N 5/10 (2006.01); C12N 9/78 (2006.01); C12N 15/09 (2006.01); C12N 15/85 (2006.01); C12P 21/00 (2006.01); C12R 1/91 (2006.01)
CPC (source: EP)
C12N 9/78 (2013.01); C12N 15/85 (2013.01)
Citation (search report)
- [Y] EP 0117060 A2 19840829 - GENENTECH INC [US]
- [Y] FEDERATION PROCEEDINGS, vol. 44, no. 3, 1st March 1985, page 667, abstract 1502, Washington, D.C., US; J. PFEILSTICKER et al.: "Construction of a mammalian expression vector containing a cDNA for rat adenosine deaminase"
- [AD] JOURNAL OF BIOLOGICAL CHEMISTRY, vol. 258, no. 13, 10th July 1983, pages 8338-8345, Washington, D.C., US; C.-Y. YEUNG et al.: "Selective overproduction of adenosine deaminase in cultured mouse cells"
- [T] PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCE, vol. 83, May 1986, pages 3136-3140, Washington, D.C., US; R.J. KAUFMAN et al.: "Selection and amplification of heterologous genes encoding adenosine deaminase in mammalian cells"
- See references of WO 8606409A1
Designated contracting state (EPC)
AT BE CH DE FR GB IT LI LU NL SE
DOCDB simple family (publication)
WO 8606409 A1 19861106; EP 0221955 A1 19870520; EP 0221955 A4 19881222; JP S62502662 A 19871015
DOCDB simple family (application)
US 8600934 W 19860428; EP 86903076 A 19860428; JP 50263886 A 19860428