EP 0232410 A4 19880125 - LYSIS METHOD AND BUFFER FOR EXTRACTION OF HEPATITIS B SURFACE ANTIGEN FROM YEAST CELLS.
Title (en)
LYSIS METHOD AND BUFFER FOR EXTRACTION OF HEPATITIS B SURFACE ANTIGEN FROM YEAST CELLS.
Title (de)
SPALTVERFAHREN UND PUFFER ZUR EXTRAKTION VON HEPATITIS-B-OBERFLÄCHENANTIGEN AUS HEFEZELLEN.
Title (fr)
PROCEDE ET TAMPON DE LYSE POUR L'EXTRACTION D'UN ANTIGENE DE SURFACE DE L'HEPATITE B A PARTIR DE CELLULES DE LEVURE.
Publication
Application
Priority
US 76604385 A 19850815
Abstract (en)
[origin: WO8701128A1] A yeast cell lysis buffer for enhancing the recovery of subcellular particles including an aqueous solution of a nonionic detergent at a concentration within the range of about 0.5% to about 1.0% by volume at a temperature within a range from about 3<o>C to about 8<o>C. Triton X-100 <R> is a preferred nonionic detergent for use in the lysis buffer. This lysis buffer may be employed in a method of extracting subcellular particles (e.g. HBsAg particles) from cells (e.g. genetically transformed yeast cells) which are broken open in the presence of the lysis buffer.
IPC 1-7
IPC 8 full level
A61K 39/29 (2006.01); C07K 14/005 (2006.01); C07K 14/02 (2006.01); C07K 14/10 (2006.01); C07K 14/18 (2006.01); C07K 14/195 (2006.01); C12N 1/06 (2006.01); C12N 15/09 (2006.01); C12P 21/00 (2006.01); C12R 1/865 (2006.01)
CPC (source: EP)
C07K 14/005 (2013.01); C12N 1/063 (2013.01); C12N 2730/10122 (2013.01)
Citation (search report)
- [E] EP 0204680 A2 19861210 - SMITH KLINE RIT [BE]
- [Y] EP 0110385 A2 19840613 - US HEALTH [US]
- [Y] EP 0075395 A2 19830330 - BIOGEN NV [NL]
- See references of WO 8701128A1
Designated contracting state (EPC)
AT BE CH DE FR GB IT LI LU NL SE
DOCDB simple family (publication)
WO 8701128 A1 19870226; EP 0232410 A1 19870819; EP 0232410 A4 19880125; JP S63501121 A 19880428
DOCDB simple family (application)
US 8601704 W 19860815; EP 86905526 A 19860815; JP 50454086 A 19860815