Global Patent Index - EP 0239631 A4

EP 0239631 A4 19890112 - RECOMBINANT APOLIPOPROTEINS AND METHODS.

Title (en)

RECOMBINANT APOLIPOPROTEINS AND METHODS.

Title (de)

REKOMBINANTE APOLIPOPROTEINE UND VERFAHREN.

Title (fr)

APOLIPOPROTEINES RECOMBINANTES ET PROCEDES.

Publication

EP 0239631 A4 19890112 (EN)

Application

EP 86906545 A 19861002

Priority

  • US 78441885 A 19851004
  • US 80469285 A 19851204

Abstract (en)

[origin: WO8702062A1] A method of producing a purified lipid-binding peptide which can bind to phospholipids at one or more amphipatic alpha-helical peptide regions. The method includes providing a gene coding for the peptide, and introducing the gene in expressible, heterologous form in a suitable expression system capable of synthesizing a mixture of peptides which includes the lipid-binding peptide. Addition of either endogenous or exogenous lipids to the peptide mixture forms a low-density lipopeptide complex composed of lipid and the lipid-binding peptide, and this complex can be separated easily from nonlipid-binding peptides in the peptide on the basis of its size and/or density. The method is intended particularly for scaled-up production of purified human apolipoproteins and their alpha-helical lipid-binding regions. Also disclosed are related methods for producing recombinant apolipoproteins, therapeutic lipopeptide compositions, and a stabilized lipid emulsion for nutritional therapy. Further disclosed are methods for expressing apolipoproteins or lipid-binding segments thereof in bacterial, yeast and mammalian cell expression systems, and methods for purifying lipid binding proteins, including fused recombinant proteins.

IPC 1-7

C12P 21/00; C12N 15/00; A61K 37/02; C07K 13/00; C07H 17/00

IPC 8 full level

A61K 31/00 (2006.01); C07K 14/775 (2006.01); C12N 15/62 (2006.01); C12N 15/85 (2006.01); C12P 21/02 (2006.01)

CPC (source: EP)

A61K 31/00 (2013.01); C07K 14/775 (2013.01); C12N 15/62 (2013.01); C12N 15/85 (2013.01); C12P 21/02 (2013.01); C07K 2319/00 (2013.01); C07K 2319/02 (2013.01); C07K 2319/75 (2013.01)

Citation (search report)

  • [XP] WO 8604920 A1 19860828 - BIOTECH RES PARTNERS LTD [US]
  • [E] WO 8702061 A1 19870409 - BIOTECH RES PARTNERS LTD [US]
  • [A] EP 0173280 A1 19860305 - BIO TECHNOLOGY GENERAL CORP [US]
  • [XP] PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCE, USA, vol. 83, March 1986, pages 1467-1471, Washington, DC, US; A.A. PROTTER et al.: "Isolation of a cDNA clone encoding the amino-terminal region of human apolipoprotein B"
  • [T] JOURNAL OF BIOLOGICAL CHEMISTRY, vol. 262, no. 9, March 1987, pages 4241-4247, The American Society of Biological Chemists, Inc., Washington, DC, US; J. BEDNARZ MALLORY et al.: "Expression and characterization of human apolipoprotein A-I in Chinese hamster ovary cells"
  • [X] JOURNAL OF MEDICINAL CHEMISTRY, vol. 25, no. 10, October 1982, pages 1115-1120, American Chemical Society, Washington, DC, US; R.E. COUNSELL et al.: "Lipoproteins as potential site-specific delivery systems for diagnostic and therapeutic agents"
  • See references of WO 8702062A1

Designated contracting state (EPC)

AT BE CH DE FR GB IT LI LU NL SE

DOCDB simple family (publication)

WO 8702062 A1 19870409; AU 6522486 A 19870424; EP 0239631 A1 19871007; EP 0239631 A4 19890112

DOCDB simple family (application)

US 8602075 W 19861002; AU 6522486 A 19861002; EP 86906545 A 19861002