Global Patent Index - EP 0260299 A4

EP 0260299 A4 19881124 - MUTAGENESIS AND SCREENING METHOD AND PRODUCT.

Title (en)

MUTAGENESIS AND SCREENING METHOD AND PRODUCT.

Title (de)

VERFAHREN ZUR MUTAGENESIS UND ÜBERPRÜFUNG UND PRODUKT.

Title (fr)

PROCEDE DE MUTAGENESE ET DE CRIBLAGE ET PRODUIT OBTENU.

Publication

EP 0260299 A4 19881124 (EN)

Application

EP 87901901 A 19870212

Priority

US 82854586 A 19860212

Abstract (en)

[origin: WO8705050A1] Cloned DNA is mutated by creating a single-stranded target region in a cloned DNA segment, and introducing a mutation into the single-stranded target region by treating the target region with a chemical or biological mutagenizing agent capable of introducing mutations into single-stranded DNA. The mutated target region then is rendered double-stranded and a microorganism is transformed with the mutated double-stranded DNA present in an expression vector. The transformed microorganism is cultivated under conditions wherein the mutated DNA is expressed to form an expression product, and the expression product is screened to identify a desired mutation in the DNA segment. Mutant subtilisins of enhanced thermal stability are also disclosed.

IPC 1-7

C07H 21/00; C12N 1/20; C12N 9/56; C12N 15/00; C12P 21/00; C12Q 1/68; D06M 16/00

IPC 8 full level

C07K 14/00 (2006.01); C07K 14/195 (2006.01); C07K 14/41 (2006.01); C11D 3/386 (2006.01); C12N 1/20 (2006.01); C12N 9/54 (2006.01); C12N 9/56 (2006.01); C12N 15/00 (2006.01); C12N 15/01 (2006.01); C12N 15/09 (2006.01); C12N 15/10 (2006.01); C12P 21/02 (2006.01); C12Q 1/37 (2006.01); C12Q 1/68 (2006.01); G01N 33/50 (2006.01); C12R 1/125 (2006.01)

CPC (source: EP)

C11D 3/386 (2013.01); C12N 9/54 (2013.01); C12N 15/102 (2013.01); C12Q 1/37 (2013.01); C12Q 1/68 (2013.01)

Citation (search report)

  • [X] EP 0138075 A1 19850424 - SYNERGEN ASSOCIATES INC [US]
  • [Y] FR 2423541 A1 19791116 - PASTEUR INSTITUT [FR]
  • [A] EP 0108303 A1 19840516 - MILES LAB [US]
  • [E] WO 8704461 A1 19870730 - AMGEN [US]
  • [XO] J. CELL. BIOCHEM., SUPPL., no. 10, part A, 1986, page 271, no. E101; P. BRYAN et al.: "Protein engineering of subtilisin: proteases of enhanced stability", & SYMPOSIUM ON PROTEASES IN BIOLOGICAL CONTROL AND BIOTECHNOLOGY, 15TH ANNUAL UCLA, Los Angeles, 9th-15th February 1986
  • [X] PROC. NATL. ACAD. SCI. USA, vol. 82, February 1985, pages 747-750; T. ALBER et al.: "A genetic screen for mutations that increase the thermal stability of phage T4 lysozyme"
  • [X] THE JOURNAL OF BIOLOGICAL CHEMISTRY, vol. 260, 5th December 1985, pages 15298-15303, The American Society of Biological Chemists, Inc., US; M. MATSUMURA et al.: "Screening for thermostable mutant of kanamycin nucleotidyltransferase by the use of a transformation system for a thermophile, Bacillus stearothermophilus"
  • THE JOURNAL OF BIOLOGICAL CHEMISTRY, vol. 260, 05 December 1985, The American Society of Biological Chemists Inc., US; M. MATSUMURA et al., pp. 15298-15303#
  • [XP] PROTEINS: STRUCTURE, FUNCTION AND GENETICS, vol. 1, 10th November 1986, pages 326-334; P.N. BRYAN et al.: "Proteases of enhanced stability: characterization of a thermostable variant of subtilisin"
  • [A] SCIENCE, vol. 229, no. 4719, 20th September 1985, pages 1193-1201; D. BOTSTEIN et al.: "Strategies and applications of in vitro mutagenesis"
  • [XP] GENETIC TECHNOLOGY NEWS, vol. 6, no. 5, May 1986, pages 3-4; "Genex engineers detergent enzymes"
  • See references of WO 8705050A1

Designated contracting state (EPC)

AT BE CH DE FR GB IT LI LU NL SE

DOCDB simple family (publication)

WO 8705050 A1 19870827; DK 531887 A 19871012; DK 531887 D0 19871012; EP 0260299 A1 19880323; EP 0260299 A4 19881124; JP S63502959 A 19881102

DOCDB simple family (application)

US 8700348 W 19870212; DK 531887 A 19871012; EP 87901901 A 19870212; JP 50162187 A 19870212