EP 0265785 A2 19880504 - Polypeptides derived from the envelope gene of human immunodeficiency virus in recombinant baculovirus infected insect cells.
Title (en)
Polypeptides derived from the envelope gene of human immunodeficiency virus in recombinant baculovirus infected insect cells.
Title (de)
Von der Hülle des Virus des erworbenen Immunmangelsyndroms abgeleitete Polypeptide in von rekombinanten Bakuloviren infizierten Zellen.
Title (fr)
Polypeptides dérivés du gène d'enveloppe du virus d'immuno-dépression acquise, dans des cellules d'insectes infectées par un baculovirus recombinant.
Publication
Application
Priority
US 92019786 A 19861016
Abstract (en)
The baculovirus-insect cell vector system is used as a high efficiency eukaryotic cloning and expression method for the production of recombinant proteins. A major application of this system is also in the development of rDNA vaccines for a variety of important human and animal diseases. This system is used to express a variety of candidate vaccine immunogens derived from the envelope gene of Human Immunodeficiency Virus (HIV). The envelope protein of HIV is the most promising candidate in the development of vaccines and diagnostics for AIDS since it has been shown that the envelope glycoprotein represents the major target antigen of AIDS virus. Recombinant baculoviruses have been constructed which express a variety of domains of the envelope gene (env), including gp 160 and gp 120. Expression of the recombinant HIV baculoviruses in insect cells results in the production of glycoproteins which are reactive with HIV-specific serum. HIV antigens produced from some of these recombinants have been successfully employed in a variety of immunodiagnostic assays including ELISA, Western Blotting and radioimmunoprecipitation.
IPC 1-7
A61K 39/21; C07K 1/14; C12N 7/00; C12N 15/00; C12P 21/00; G01N 33/569
IPC 8 full level
C12N 15/09 (2006.01); A61K 39/21 (2006.01); C07K 1/22 (2006.01); C07K 14/00 (2006.01); C07K 14/005 (2006.01); C07K 14/155 (2006.01); C07K 14/16 (2006.01); C07K 14/195 (2006.01); C07K 19/00 (2006.01); C12N 15/48 (2006.01); C12N 15/86 (2006.01); C12N 15/866 (2006.01); C12P 21/00 (2006.01); G01N 33/569 (2006.01); G01N 33/577 (2006.01); A61K 39/00 (2006.01); C12R 1/91 (2006.01)
CPC (source: EP)
C07K 14/005 (2013.01); C12N 15/86 (2013.01); A61K 39/00 (2013.01); C12N 2710/14143 (2013.01); C12N 2740/16222 (2013.01); C12N 2740/16234 (2013.01); C12N 2810/6054 (2013.01); C12N 2830/00 (2013.01); C12N 2830/60 (2013.01)
Designated contracting state (EPC)
AT BE CH DE ES FR GB GR IT LI LU NL SE
DOCDB simple family (publication)
EP 0265785 A2 19880504; EP 0265785 A3 19891108; AR 241940 A1 19930129; AU 7984287 A 19880421; AU 8832491 A 19920430; BR 8705535 A 19880524; CN 87107837 A 19881221; DD 269629 A5 19890705; DD 283935 A5 19901031; DD 284046 A5 19901031; DK 541987 A 19880417; DK 541987 D0 19871016; FI 874564 A0 19871016; FI 874564 A 19880417; HU T45095 A 19880530; IE 872748 L 19880416; IL 84154 A0 19880331; JP S63207397 A 19880826; LT IP1792 A 19950825; LT IP1793 A 19950825; LT IP1794 A 19950825; LV 10654 A 19950420; LV 10654 B 19951020; PL 161165 B1 19930531; PL 161448 B1 19930630; PL 268266 A1 19880818; ZA 877752 B 19890628
DOCDB simple family (application)
EP 87115085 A 19871015; AR 30898187 A 19871016; AU 7984287 A 19871016; AU 8832491 A 19911129; BR 8705535 A 19871016; CN 87107837 A 19871016; DD 30803987 A 19871016; DD 32944087 A 19871016; DD 32944187 A 19871016; DK 541987 A 19871016; FI 874564 A 19871016; HU 466687 A 19871016; IE 274887 A 19871013; IL 8415487 A 19871013; JP 26257287 A 19871016; LT IP1792 A 19940124; LT IP1793 A 19940124; LT IP1794 A 19940124; LV 930344 A 19930514; PL 26826687 A 19871016; PL 29352087 A 19871016; ZA 877752 A 19871015