EP 0266414 A4 19891228 - METHOD FOR PREPARING CONTACT-INHIBITORY FACTOR.
Title (en)
METHOD FOR PREPARING CONTACT-INHIBITORY FACTOR.
Title (de)
VERFAHREN ZUR HERSTELLUNG EINES KONTAKTINHIBIERENDEN FAKTORS.
Title (fr)
PROCEDE DE PREPARATION D'UN FACTEUR D'INHIBITION PAR CONTACT.
Publication
Application
Priority
US 85604086 A 19860423
Abstract (en)
[origin: WO8706589A1] A method for preparing a biologically active factor that is capable of restoring contact-inhibition of growth in malignant cell type. The method comprises loading a mixture comprising culture medium conditioned by growth of a contact-inhibited cell culture and an ionic buffer to a chromatography column equilibrated with the same buffer and comprising a non-ionic hydrophobic affinity material as the adsorbant. The column is washed once with the same buffer until a peak of material elutes, a second time with the same buffer with decreased ionic strength which lacks sodium chloride until a second peak elutes, and eluting the factor with unbuffered purified water. The eluate is desalted by membrane filtration and dried. The method is conducted in the absence of a volatile precipitating agent.
IPC 1-7
IPC 8 full level
G01N 30/88 (2006.01); A61K 9/00 (2006.01); A61K 35/12 (2006.01); A61K 35/13 (2015.01); C07K 1/20 (2006.01); C07K 14/005 (2006.01); C07K 14/195 (2006.01); C12N 15/09 (2006.01); C12P 1/00 (2006.01); C12P 21/00 (2006.01); C12R 1/91 (2006.01)
CPC (source: EP)
A61K 9/127 (2013.01); A61K 35/13 (2013.01); A61K 45/06 (2013.01); C12N 1/00 (2013.01); C12P 1/00 (2013.01); C12R 2001/91 (2021.05)
Citation (search report)
- No relevant documents have been disclosed.
- See references of WO 8706589A1
Designated contracting state (EPC)
AT BE CH DE FR GB IT LI LU NL SE
DOCDB simple family (publication)
WO 8706589 A1 19871105; EP 0266414 A1 19880511; EP 0266414 A4 19891228; JP S63503113 A 19881117
DOCDB simple family (application)
US 8700928 W 19870421; EP 87903511 A 19870421; JP 50300787 A 19870421