EP 0274527 B1 19940504 - USE OF BACTERIAL LUCIFERASE STRUCTURAL GENES FOR CLONING AND MONITORING GENE EXPRESSION IN MICROORGANISMS AND FOR TAGGING AND IDENTIFICATION OF GENETICALLY ENGINEERED ORGANISMS.
Title (en)
USE OF BACTERIAL LUCIFERASE STRUCTURAL GENES FOR CLONING AND MONITORING GENE EXPRESSION IN MICROORGANISMS AND FOR TAGGING AND IDENTIFICATION OF GENETICALLY ENGINEERED ORGANISMS.
Title (de)
VERWENDUNG VON BAKTERIELLEN LUCIFERASE STRUKTURELLEN GENEN ZUM KLONIEREN UND ZUR STEUERUNG DER GENEXPRESSION IN MIKROORGANISMEN, SOWIE ZUR ETIKETTIERUNG UND IDENTIFIZIERUNG VON GENETISCH GEBILDETEN ORGANISMEN.
Title (fr)
UTILISATION DE GENES STRUCTURAUX DE LUCIFERASE BACTERIENNE POUR LE CLONAGE ET LE CONTROLE DE L'EXPRESSION GENETIQUE DANS DES MICROORGANISMES ET POUR LE MARQUAGE ET L'IDENTIFICATION D'ORGANISME MODIFIES PAR GENIE GENETIQUE.
Publication
Application
Priority
- US 8701730 W 19870721
- US 88874686 A 19860722
Abstract (en)
[origin: WO8800617A1] A host microorganism is genetically and stably modified by the insertion in any of its non-essential chromosomal locations a foreign DNA fragment comprising a non-homologous fragment containing a lux AB gene of a selected bioluminescent bacterium such as V. harveyi such that the nonhomologous fragment is stably reproduced as a part of the chromosome of the cells of the host microorganism as they divide in a growth medium and the gene expression of such microorganism triggers the production of luciferase which, in turn emits light in the presence of aldehyde as a measure of gene expression. Both Gram-positive and Gram-negative bacteria are microorganisms which may be modified as aforesaid. X-ray film can be used to quantify the light being emitted from a microorganism through the use of plural droplets containing the same microorganism each with a known and related cell (or plasmid) count.
IPC 1-7
IPC 8 full level
C12N 9/02 (2006.01); C12N 15/90 (2006.01); C12Q 1/06 (2006.01); C12Q 1/68 (2006.01)
CPC (source: EP)
C12N 9/0071 (2013.01); C12N 15/90 (2013.01); C12Q 1/06 (2013.01); C12Q 1/6897 (2013.01); C12Y 114/14003 (2013.01)
Citation (examination)
JOURNAL OF BACTERIOLOGY, vol. 167, issued 02 July 1986, Washington, D.C., SCHMETTERER et al., pp. 411-414;
Designated contracting state (EPC)
AT BE CH DE FR GB IT LI LU NL SE
DOCDB simple family (publication)
WO 8800617 A1 19880128; AU 7751687 A 19880210; CA 1333776 C 19950103; DE 3789766 D1 19940609; DE 3789766 T2 19940929; EP 0274527 A1 19880720; EP 0274527 A4 19901128; EP 0274527 B1 19940504
DOCDB simple family (application)
US 8701730 W 19870721; AU 7751687 A 19870721; CA 542601 A 19870721; DE 3789766 T 19870721; EP 87905086 A 19870721