EP 0289586 A4 19900410 - ENHANCING GAMMA-CARBOXYLATION OF RECOMBINANT VITAMIN K-DEPENDENT PROTEINS.
Title (en)
ENHANCING GAMMA-CARBOXYLATION OF RECOMBINANT VITAMIN K-DEPENDENT PROTEINS.
Title (de)
STEIGERUNG DER GAMMA-CARBOXYLATION VON REKOMBINANTEN, VITAMIN-K-ABHÄNGIGEN PROTEINEN.
Title (fr)
AMELIORATION DE LA GAMMA-CARBOXYLATION DE PROTEINES RECOMBINANTES DEPENDANTES DE LA VITAMINE K.
Publication
Application
Priority
US 93165186 A 19861117
Abstract (en)
[origin: WO8803926A1] A DNA sequence including a first DNA sequence encoding a human vitamin K-dependent protein having fused to its 5' end a second DNA sequence not identical to the propeptide encoding sequence naturally associated with the DNA sequence encoding the protein, the non-naturally occuring propeptide encoding sequence being capable of encoding a propeptide which is capable of enhancing the gamma-carboxylation of the protein when the protein is expressed in a recombinant eukaryotic cell.
IPC 1-7
IPC 8 full level
C12N 15/09 (2006.01); C07H 21/04 (2006.01); C12N 9/74 (2006.01); C12N 15/62 (2006.01); C12P 21/00 (2006.01); C12R 1/91 (2006.01)
CPC (source: EP)
C12N 9/6429 (2013.01); C12N 15/62 (2013.01); C12Y 304/21005 (2013.01); C07K 2319/00 (2013.01); C07K 2319/02 (2013.01)
Citation (search report)
- [Y] EP 0195592 A2 19860924 - NAT RES DEV [GB]
- [E] EP 0266190 A2 19880504 - ZYMOGENETICS INC [US]
- [XP] EP 0200421 A2 19861105 - ZYMOGENETICS INC [US]
- [XD] BIOCHEMISTRY, vol. 22, 1983, pages 2087-2097, American Chemical Society; S.J. FRIEZNER et al.: "Characterization of the complementary deoxyribonucleic acid and gene coding for human prothrombin"
- [AD] THE JOURNAL OF BIOLOGICAL CHEMISTRY, vol. 261, no. 21, 25th July 1986, pages 9622-9628, The American Society of Biological Chemists, Inc.; R.J. KAUFMAN et al.: "Expression, Purification, and characterization of recombinant y-carboxylated factor IX synthesized in chinese hamster ovary cells"
- See references of WO 8803926A1
Designated contracting state (EPC)
AT BE CH DE FR GB IT LI LU NL SE
DOCDB simple family (publication)
WO 8803926 A1 19880602; AU 8339187 A 19880616; EP 0289586 A1 19881109; EP 0289586 A4 19900410; JP H01502080 A 19890727
DOCDB simple family (application)
US 8703015 W 19871117; AU 8339187 A 19871117; EP 88900051 A 19871117; JP 50045588 A 19871117