EP 0295965 A2 19881221 - Oscillator-based methods of detecting a member of a specific binding pair.
Title (en)
Oscillator-based methods of detecting a member of a specific binding pair.
Title (de)
Auf einem Oszillator basiertes Verfahren zum Detektieren eines Mitgliedes eines spezifischen Bindungspaares.
Title (fr)
Procédé pour déterminer un membre d'une paire de liaison spécifique basé sur un oscillateur.
Publication
Application
Priority
US 6426687 A 19870618
Abstract (en)
A method for detecting a first member of a specific binding pair in a sample, the first member being capable of forming a complex with a second member of the specific binding pair, includes immobilizing the second member on a surface of a piezo-electric crystal oscillator, determining a first vibrational frequency of the oscillator on which the second member has been immobilized, contacting the surface of the oscillator on which the second member has been immobilized with the sample under conditions permitting formation of the complex, and with a mass increasing particle under conditions permitting binding of the particle with the first member, mass coupling the bound particle with the oscillator, and determining a second vibrational frequency of the oscillator with which the particle has been mass coupled, the difference between the first vibrational frequency and the second vibrational frequency providing a measure of the quantity of the first member in the sample. In an alternative method a particle binding member is used, having two binding regions capable of binding to the first member and the particle, respectively. Because the mass of each particle is relatively greater than that of the member, the mass coupling on the surface effectively amplifies the mass increase owing to the specific complexing.
IPC 1-7
IPC 8 full level
C12Q 1/68 (2006.01); C12Q 1/6825 (2018.01); C12Q 1/689 (2018.01); G01N 27/00 (2006.01); G01N 33/543 (2006.01)
CPC (source: EP US)
C12Q 1/6825 (2013.01 - EP US); C12Q 1/689 (2013.01 - EP US); G01N 29/036 (2013.01 - EP US); G01N 33/54373 (2013.01 - EP US); G01N 2291/0256 (2013.01 - EP US); G01N 2291/0257 (2013.01 - EP US); Y10S 436/806 (2013.01 - EP US)
Designated contracting state (EPC)
BE DE FR GB GR IT LU NL
DOCDB simple family (publication)
EP 0295965 A2 19881221; EP 0295965 A3 19910417; EP 0295965 B1 19940622; DE 3850305 D1 19940728; DE 3850305 T2 19941222; DK 333888 A 19881219; DK 333888 D0 19880617; JP S6435269 A 19890206; PT 87739 A 19880701; PT 87739 B 19921030; US 4847193 A 19890711
DOCDB simple family (application)
EP 88305597 A 19880620; DE 3850305 T 19880620; DK 333888 A 19880617; JP 15107288 A 19880618; PT 8773988 A 19880616; US 6426687 A 19870618