EP 0301333 A2 19890201 - Liposome based homogeneous immunoassay for diagnostic tests.
Title (en)
Liposome based homogeneous immunoassay for diagnostic tests.
Title (de)
Auf liposomen basierter, homogener Immunoassay für diagnostische Teste.
Title (fr)
Essai immunologique à base de liposomes pour des tests diagnostiques.
Publication
Application
Priority
US 8591887 A 19870729
Abstract (en)
The present invention provides for novel homogeneous immunoassay systems involving complement-mediated lysis of marker-encapsulating lipid vesicles (liposomes) for detection of analyte in a fluid sample. Antibody sensitized liposomes (the first reagent) are sequentially incubated with an analyte-containing sample, and optionally "dummy" liposomes, which do not contain encapsulated marker, a second antibody (the second reagent), and finally with a complement source such as plasma. Complement is activated by the liposome-antibody-antigen-second antibody complex causing liposome lysis and a concomitant release of marker. Antibody of the first reagent may be an anti-analyte F(ab min )2 antibody fragment, or an anti-analyte Fab min antibody fragment. Antibody of the second reagent may be provided in either soluble form, or in insoluble form e.g., bound onto carboxylated polystyrene particles or coupled to a third antibody in the form of a "double antibody" immune precipitate. Also provided are methods for preparing antibody sensitized liposomes in the presence of a polysaccharide capable of forming a reversible gel and methods for preparing derivatized Fab min antibody fragments for coupling to lipid vesicles.
IPC 1-7
IPC 8 full level
G01N 33/542 (2006.01); G01N 33/543 (2006.01); G01N 33/544 (2006.01); G01N 33/58 (2006.01); G01N 33/68 (2006.01); G01N 33/76 (2006.01)
CPC (source: EP KR)
G01N 33/48 (2013.01 - KR); G01N 33/5432 (2013.01 - EP); G01N 33/586 (2013.01 - EP); G01N 33/6857 (2013.01 - EP); G01N 33/76 (2013.01 - EP)
Designated contracting state (EPC)
AT BE CH DE ES FR GB IT LI NL
DOCDB simple family (publication)
EP 0301333 A2 19890201; EP 0301333 A3 19920701; AU 2006988 A 19890202; AU 620554 B2 19920220; CA 1309344 C 19921027; JP S6450963 A 19890227; KR 890002664 A 19890411
DOCDB simple family (application)
EP 88111397 A 19880715; AU 2006988 A 19880727; CA 573112 A 19880727; JP 19198488 A 19880728; KR 880009438 A 19880727