EP 0307149 B1 19930107 - SOLID PHASE IMMUNOASSAY FOR AN ANTIBODY AND BIOLOGICAL CONSTRUCTIONS FOR USE THEREIN
Title (en)
SOLID PHASE IMMUNOASSAY FOR AN ANTIBODY AND BIOLOGICAL CONSTRUCTIONS FOR USE THEREIN
Publication
Application
Priority
- GB 8720800 A 19870904
- GB 8818030 A 19880728
Abstract (en)
[origin: EP0307149A2] An immunoassay for an antibody comprises: (i) contacting a solid phase, on which is immobilised a first recombinant peptide which presents an antigenic sequence to which the antibody is capable of binding, with a test sample; (ii) contacting the solid phase with a second recombinant peptide which presents the said antigenic sequence, which is labelled and which was expressed in an organism of a different genus than that in which the first recombinant peptide was expressed; and (iii) determining whether the test sample contained any said antibody. A suitable protein for use in assaying for anti-p24 and anti-gp41 HIV-1 antibody is a fusion of a gag sequence comprising amino acids 121-356 and an env sequence comprising amino acids 542-674. The amino acids are numbered according to Meusing et al, Nature, 313, 450-458 (1985).
IPC 1-7
A61K 39/21; C07K 13/00; C12N 15/00; C12P 21/02; G01N 33/543; G01N 33/569
IPC 8 full level
G01N 33/569 (2006.01); A61K 38/00 (2006.01); C07K 14/16 (2006.01); C12N 15/09 (2006.01); C12P 21/02 (2006.01); G01N 33/577 (2006.01); C12R 1/91 (2006.01)
CPC (source: EP)
C07K 14/005 (2013.01); C07K 2319/00 (2013.01); C07K 2319/40 (2013.01); C07K 2319/735 (2013.01); C12N 2740/16122 (2013.01)
Citation (examination)
CURRENT TOPICS IN AIDS, vol. 1, 1987, John Wiley & Sons Ltd.; M.S.GOTTLIEB et al., pp. 95-102<
Designated contracting state (EPC)
AT BE CH DE ES FR GB GR IT LI LU NL SE
DOCDB simple family (publication)
EP 0307149 A2 19890315; EP 0307149 A3 19890503; EP 0307149 B1 19930107; AU 2182588 A 19890309; AU 615670 B2 19911010; CA 1341343 C 20020226; DE 3877294 D1 19930218; DE 3877294 T2 19930506; DK 490888 A 19890305; DK 490888 D0 19880902; ES 2051859 T3 19940701; IE 63455 B1 19950419; IE 882658 L 19890304; JP 2634203 B2 19970723; JP H01163665 A 19890627; NZ 226026 A 19910326; PT 88416 A 19890731; PT 88416 B 19921030
DOCDB simple family (application)
EP 88308170 A 19880902; AU 2182588 A 19880902; CA 577076 A 19880906; DE 3877294 T 19880902; DK 490888 A 19880902; ES 88308170 T 19880902; IE 265888 A 19880902; JP 22030588 A 19880902; NZ 22602688 A 19880902; PT 8841688 A 19880902