EP 0310014 B1 19950621 - Chromogene substrate.
Title (en)
Chromogene substrate.
Title (de)
Chromogene Substrate.
Title (fr)
Substrat chromogène.
Publication
Application
Priority
DE 3732871 A 19870930
Abstract (en)
[origin: US5077200A] Chromogenic substrates for the detection of hydrolyzing enzymes, processes for the preparation of these chromogenic substrates and the use of the chromogenic substrates. The quantification of hydrolyzing enzymes for diagnostic purposes has to date been carried out by determination of the amounts of a fluorescent or highly absorbent chromogenic dyestuff liberated in the hydrolytic reaction, detection of which has in part been possible exlusively by instruments or in the neutral to alkaline pH range. After enzymatic hydrolysis, the chromogenic substrates according to the invention lead to highly sensitive and specifically measurable signals regardless of the pH range. Azo dyestuff compounds with the general formula A-N=N-B (OR) in which A) and B) have the meanings given in claim 1 and R) is a radical which can be liberated by enzymatic hydrolysis, excluding a carbonyl radical, are provided. After enzymatic hydrolysis, highly sensitively and specifically measurable signals are obtained regardless of the pH range. The azo dystuff compounds mentioned are used for the detection of hydrolyzing enzymes, in particular glycosidases, phosphatases and sulfatases.
IPC 1-7
IPC 8 full level
G01N 33/50 (2006.01); C09B 29/42 (2006.01); C09B 43/18 (2006.01); C12Q 1/34 (2006.01); C12Q 1/42 (2006.01); C12Q 1/44 (2006.01); C12Q 1/46 (2006.01)
CPC (source: EP US)
C12Q 1/34 (2013.01 - EP US); C12Q 1/42 (2013.01 - EP US); C12Q 1/44 (2013.01 - EP US); C12Q 1/46 (2013.01 - EP US); C12Q 2334/00 (2013.01 - EP US); Y10S 530/802 (2013.01 - EP US)
Designated contracting state (EPC)
AT BE CH DE ES FR GB IT LI LU NL SE
DOCDB simple family (publication)
EP 0310014 A2 19890405; EP 0310014 A3 19910306; EP 0310014 B1 19950621; AT E124089 T1 19950715; AU 2295888 A 19890406; AU 608900 B2 19910418; CA 1332806 C 19941101; DE 3732871 A1 19890420; DE 3854032 D1 19950727; ES 2076153 T3 19951101; JP 2675835 B2 19971112; JP H01115962 A 19890509; US 5077200 A 19911231
DOCDB simple family (application)
EP 88115957 A 19880928; AT 88115957 T 19880928; AU 2295888 A 19880929; CA 578824 A 19880929; DE 3732871 A 19870930; DE 3854032 T 19880928; ES 88115957 T 19880928; JP 24262488 A 19880929; US 25116688 A 19880928