EP 0321479 B1 19940309 - ENZYMATIC MEMBRANE METHOD FOR THE SYNTHESIS AND SEPARATION OF PEPTIDES.
Title (en)
ENZYMATIC MEMBRANE METHOD FOR THE SYNTHESIS AND SEPARATION OF PEPTIDES.
Title (de)
ENZYMATISCHE MEMBRAN, VERFAHREN ZUR HERSTELLUNG UND TRENNUNG VON PEPTIDEN.
Title (fr)
PROCEDE A MEMBRANE ENZYMATIQUE DE SYNTHESE ET DE SEPARATION DE PEPTIDES.
Publication
Application
Priority
- US 7850487 A 19870728
- US 89767986 A 19860818
Abstract (en)
[origin: ES2007103A6] The present invention provides a membrane method for the enzymatic synthesis of peptides accomplished by shifting the chemical equilibrium that exists in a reaction mixture between charged or ionized reacting amino acids and uncharged or non-ionized peptide product in the presence of a proteolytic enzyme such as thermolysin. The equilibrium is shifted by diffusion of the unchanged peptide product across an ion-rejection membrane which removes the uncharged peptide from the reaction mixture and preferably the diffused uncharged peptide is quickly converted to a charged species that cannot back-diffuse into the reaction mixture so that the uncharged peptide is effectively "pulled" across the membrane. An enzymatic conversion of the uncharged species utilizing an esterase having proteolytic activity such as aminoacylase I is disclosed. Copermeating reactants can be separated from the product mixture and returned to the reaction mixture. Also, the ion-rejection membrane can be utilized to resolve enantiomers of racemic carboxylic acids including D,L-amino acids.
IPC 1-7
IPC 8 full level
C07K 1/14 (2006.01); C07C 227/34 (2006.01); C07K 1/00 (2006.01); C07K 1/02 (2006.01); C07K 1/12 (2006.01); C07K 5/06 (2006.01); C07K 5/072 (2006.01); C07K 5/075 (2006.01); C12M 1/14 (2006.01); C12P 21/02 (2006.01); C12P 41/00 (2006.01)
IPC 8 main group level
C12P (2006.01)
CPC (source: EP KR US)
C07K 1/00 (2013.01 - EP US); C07K 5/0613 (2013.01 - EP US); C12P 21/02 (2013.01 - EP KR US); Y10S 435/813 (2013.01 - EP US); Y10S 435/819 (2013.01 - EP US)
Designated contracting state (EPC)
AT BE CH DE FR IT LI LU NL SE
DOCDB simple family (publication)
WO 8801298 A2 19880225; WO 8801298 A3 19880407; AR 242264 A1 19930331; AT E102654 T1 19940315; AU 610520 B2 19910523; AU 7854387 A 19880308; CN 1026498 C 19941109; CN 87106791 A 19880504; DE 3789309 D1 19940414; DE 3789309 T2 19941006; DK 209388 A 19880415; DK 209388 D0 19880415; EG 18454 A 19941030; EP 0321479 A1 19890628; EP 0321479 B1 19940309; ES 2007103 A6 19890601; FI 884359 A0 19880922; FI 884359 A 19880922; GB 2218991 A 19891129; GB 2218991 B 19910213; GB 8903271 D0 19890823; GR 871290 B 19871223; IL 83564 A0 19880131; IL 83564 A 19920329; IL 98061 A 19920329; JP H06319592 A 19941122; KR 880701778 A 19881105; KR 900004070 B1 19900611; MX 168598 B 19930601; NZ 221467 A 19900726; NZ 231657 A 19910726; PH 26764 A 19920928; PT 85548 A 19870901; PT 85548 B 19900531; SG 35491 G 19910621; US 5002871 A 19910326
DOCDB simple family (application)
US 8702030 W 19870814; AR 30846287 A 19870818; AT 87905545 T 19870814; AU 7854387 A 19870814; CN 87106791 A 19870818; DE 3789309 T 19870814; DK 209388 A 19880415; EG 47887 A 19870818; EP 87905545 A 19870814; ES 8702429 A 19870818; FI 884359 A 19880922; GB 8903271 A 19890214; GR 870101290 A 19870817; IL 8356487 A 19870817; IL 9806187 A 19870817; JP 12991194 A 19940520; KR 880700343 A 19880329; MX 780887 A 19870818; NZ 22146787 A 19870817; NZ 23165787 A 19870817; PH 35671 A 19870814; PT 8554887 A 19870818; SG 35491 A 19910509; US 7850487 A 19870728