EP 0350808 B1 19951108 - Method for detecting substances with hydrolase activity.
Title (en)
Method for detecting substances with hydrolase activity.
Title (de)
Verfahren zum Nachweis von Substanzen mit Hydrolaseaktivität.
Title (fr)
Méthode pour la détection de substances avec activité hydrolase.
Publication
Application
Priority
US 21738388 A 19880711
Abstract (en)
[origin: EP0350808A2] The invention relates to a method for detecting substances with hydrolase activity in a sample by mixing the sample with a hydrolase substrate and with an oxidising agent and evaluating the resulting colour intensity, characterised in that the hydrolase substrate is at least one compound of the formula I <IMAGE> in which R<1> is hydrogen or an alkoxy group R<2> is hydrogen, halogen, an alkoxy, an aralkoxy or amino group R<3>, R<4>, R<5> and R<6>, which can be identical or different, are hydrogen, halogen, an alkyl, alkoxy, aralkoxy, carboxyl, alkylcarbonyl, alkoxycarbonyl, carbamoyl, sulpho or amino group and X is a glycosyl, phosphate or acyl radical, where the radicals R<2> and R<3>, R<3> and R<4> and R<4> and R<5>, and R<5> and R<6> can each form a closed ring system, at least one of the radicals R<1> and R<2> is hydrogen and at least one of the radicals R<1>, R<2> and R<3> is not hydrogen, to a means for carrying it out and to hydrolase substrates.
IPC 1-7
IPC 8 full level
G01N 33/573 (2006.01); C07C 69/017 (2006.01); C07F 9/09 (2006.01); C07H 15/203 (2006.01); C12Q 1/34 (2006.01); C12Q 1/42 (2006.01); C12Q 1/44 (2006.01); C12Q 1/54 (2006.01); G01N 33/58 (2006.01)
CPC (source: EP KR US)
C12Q 1/34 (2013.01 - EP); G01N 33/53 (2013.01 - KR); G01N 33/581 (2013.01 - EP); H01L 29/0847 (2013.01 - US); C12Q 2334/30 (2013.01 - EP); G01N 2333/924 (2013.01 - EP)
Designated contracting state (EPC)
AT BE CH DE ES FR GB GR IT LI LU NL SE
DOCDB simple family (publication)
EP 0350808 A2 19900117; EP 0350808 A3 19920226; EP 0350808 B1 19951108; AT E130044 T1 19951115; DD 284051 A5 19901031; DE 58909481 D1 19951214; JP H0272899 A 19900313; JP H0730107 B2 19950405; KR 900002076 A 19900228; KR 920000058 B1 19920106
DOCDB simple family (application)
EP 89112440 A 19890707; AT 89112440 T 19890707; DD 33056989 A 19890707; DE 58909481 T 19890707; JP 17728689 A 19890711; KR 890009911 A 19890711