EP 0354548 A2 19900214 - Signal enhancement in assay for an enzyme.
Title (en)
Signal enhancement in assay for an enzyme.
Title (de)
Signalerhöhung im Enzym-Nachweis.
Title (fr)
Augmentation du signal dans l'essai d'enzymes.
Publication
Application
Priority
US 23093388 A 19880811
Abstract (en)
A method for assay for an unknown enzyme suspected to be present in a liquid includes signal amplification by use of a second enzyme and a blocked modulator for the second enzyme. Unknown enzyme in the liquid removes a blocking group from the blocked modulator. The resulting modulator activates or inhibits the second enzyme which catalyzes an indicator reaction in which a substrate is converted to a product. The presence or absence of the unknown enzyme in the liquid is indicated by a signal, such as a color change or a rate of color change, associated with the indicator reaction. The concentration of the enzyme in the sample may be determined by the measurement of the signal. The invention includes a kit of materials useful for performing the method of the invention.
IPC 1-7
C07C 49/173; C07C 225/06; C07F 9/12; C12Q 1/00; C12Q 1/34; C12Q 1/42
IPC 8 full level
C12Q 1/00 (2006.01); A61B 5/00 (2006.01); C12Q 1/34 (2006.01); C12Q 1/44 (2006.01)
CPC (source: EP US)
Designated contracting state (EPC)
AT BE CH DE ES FR GB GR IT LI LU NL SE
DOCDB simple family (publication)
EP 0354548 A2 19900214; EP 0354548 A3 19910612; EP 0354548 B1 19950301; AT E119205 T1 19950315; AU 3940889 A 19900215; AU 621646 B2 19920319; DE 68921385 D1 19950406; DE 68921385 T2 19950727; DK 392489 A 19900212; DK 392489 D0 19890810; ES 2068860 T3 19950501; FI 893789 A0 19890810; FI 893789 A 19900212; FI 95485 B 19951031; FI 95485 C 19960212; GR 3015261 T3 19950630; JP H02113899 A 19900426; MY 104160 A 19940228; TW 204371 B 19930421; US 4962024 A 19901009
DOCDB simple family (application)
EP 89114719 A 19890809; AT 89114719 T 19890809; AU 3940889 A 19890808; DE 68921385 T 19890809; DK 392489 A 19890810; ES 89114719 T 19890809; FI 893789 A 19890810; GR 950400384 T 19950302; JP 20947989 A 19890811; MY PI19891096 A 19890810; TW 78106780 A 19890901; US 23093388 A 19880811