Global Patent Index - EP 0372005 A4

EP 0372005 A4 19900626 - EXPRESSION SYSTEMS FOR PREPARATION OF POLYPEPTIDES IN PROKARYOTIC CELLS.

Title (en)

EXPRESSION SYSTEMS FOR PREPARATION OF POLYPEPTIDES IN PROKARYOTIC CELLS.

Title (de)

EXPRESSIONSSYSTEME ZUR ERZEUGUNG VON POLYPEPTIDEN IN PROKARYOTISCHEN ZELLEN.

Title (fr)

SYSTEMES D'EXPRESSION DESTINES A LA PREPARATION DE POLYPEPTIDES DANS DES CELLULES PROCARYOTIQUES.

Publication

EP 0372005 A4 19900626 (EN)

Application

EP 88910220 A 19881028

Priority

  • US 11513987 A 19871030
  • US 24076888 A 19880902

Abstract (en)

[origin: WO8903886A1] Expression cassettes for enhanced expression and production of a polypeptide of interest in prokaryotic cells are provided. The expression cassettes provide for production of the polypeptide of interest so that such polypeptide can either be secreted from the host cell in an active conformation or conveniently processed and renatured to a functional state. Preferably, the polypeptide of interest is expressed as a fusion protein, particularly fused to a leader sequence from a highly expressed bacterial or bacteriophage gene. The polypeptide of interest may subsequently be cleaved from the leader sequence and refolded, or used as a fusion protein.

IPC 1-7

C07H 15/12; C07K 13/00; C12N 1/20; C12N 15/00; C12P 19/34; C12P 21/00

IPC 8 full level

C12N 15/09 (2006.01); C07K 14/07 (2006.01); C07K 14/475 (2006.01); C07K 14/495 (2006.01); C07K 14/52 (2006.01); C12N 1/21 (2006.01); C12N 15/62 (2006.01); C12N 15/67 (2006.01); C12N 15/70 (2006.01); C12P 21/00 (2006.01); A61K 38/00 (2006.01); C12R 1/19 (2006.01)

CPC (source: EP)

C07K 14/005 (2013.01); C07K 14/475 (2013.01); C07K 14/495 (2013.01); C07K 14/522 (2013.01); C12N 15/62 (2013.01); C12N 15/67 (2013.01); C12N 15/70 (2013.01); A61K 38/00 (2013.01); C07K 2319/00 (2013.01); C07K 2319/02 (2013.01); C07K 2319/50 (2013.01); C07K 2319/75 (2013.01); C12N 2710/24122 (2013.01)

Citation (search report)

Designated contracting state (EPC)

AT BE CH DE FR GB IT LI LU NL SE

DOCDB simple family (publication)

WO 8903886 A1 19890505; EP 0372005 A1 19900613; EP 0372005 A4 19900626; JP 2970811 B2 19991102; JP H04502851 A 19920528

DOCDB simple family (application)

US 8803872 W 19881028; EP 88910220 A 19881028; JP 50939888 A 19881028