EP 0380508 A4 19910403 - IMPROVED PROTEIN C MOLECULES AND METHOD FOR MAKING AND ACTIVATING SAME
Title (en)
IMPROVED PROTEIN C MOLECULES AND METHOD FOR MAKING AND ACTIVATING SAME
Publication
Application
Priority
- US 8802083 W 19880617
- US 5136687 A 19870518
Abstract (en)
[origin: WO8912685A1] A method of producing recombinant human Protein C, the method including providing a vector including a DNA sequence encoding human Protein C under the transcriptional control of an eukaryotic metallothionein promoter, the vector further including at least the 69 % transforming region of the bovine papilloma virus; transfecting host eukaryotic cells with the vector; adhering the transfected cells to carrier particles; culturing the carrier particle-bound cells in culture medium to produce the recombinant Protein C; and isolating the recombinant Protein C from the culture medium or the cells. The Protein C is ideally activated by contacting same with thrombin bound to Sepharose beads in low salt concentration. Protein C produced by C127 cells following the above method are unique in that they possess an alpha -1-3 galactose linkage embueing the protein with longer in vivo half-life.
IPC 1-7
IPC 8 full level
C12N 9/64 (2006.01); C12N 11/00 (2006.01); C12N 15/00 (2006.01); C12N 15/09 (2006.01); C12N 15/12 (2006.01); C12N 15/58 (2006.01); C12N 15/85 (2006.01); C12P 21/02 (2006.01); C12R 1/91 (2006.01)
CPC (source: EP)
C12N 9/6464 (2013.01); C12Y 304/21069 (2013.01); C12N 2710/20043 (2013.01)
Citation (search report)
- [Y] EP 0323149 A2 19890705 - LILLY CO ELI [US]
- [Y] EP 0245949 A2 19871119 - LILLY CO ELI [US]
- [Y] EP 0247843 A2 19871202 - LILLY CO ELI [US]
- [Y] PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE USA, vol. 81, no. 16, August 1984, pages 5086-5090, Washington, US; R. FUKUNAGA et al.: "Constitutive production of human interferons by mouse cells with bovine papillomavirus as a vector"
- See references of WO 8912685A1
Designated contracting state (EPC)
AT BE CH DE FR GB IT LI LU NL SE
DOCDB simple family (publication)
WO 8912685 A1 19891228; DK 41590 A 19900411; DK 41590 D0 19900216; EP 0380508 A1 19900808; EP 0380508 A4 19910403; JP H03501921 A 19910509
DOCDB simple family (application)
US 8802083 W 19880617; DK 41590 A 19900216; EP 88907471 A 19880617; JP 50681388 A 19880617