EP 0381450 A2 19900808 - Assay for bone alkaline phosphatase.
Title (en)
Assay for bone alkaline phosphatase.
Title (de)
Test für die alkalische Phosphatase aus Knochen.
Title (fr)
Essai pour la phosphatase alcaline de l'os.
Publication
Application
Priority
US 30452189 A 19890131
Abstract (en)
A process for the determination of the presence or concentration of bone alkaline phosphatase (BAP) enzyme in a fluid which comprises: (a) contacting a sample of the fluid with a first monoclonal antibody for BAP, wherein the first monoclonal antibody is bound to a solid carrier insoluble in the fluid, in order to form an insoluble complex between the first monoclonal antibody and BAP; (b) separating the fluid sample containing unreacted BAP from the insoluble complex of the first monoclonal antibody and BAP; (c) reacting a measured amount of a second, labeled monoclonal antibody to BAP, which antibody is soluble in the fluid, with the insoluble complex of the first monoclonal antibody and BAP, in order to form an insoluble complex of the first monoclonal antibody, BAP, and second, labeled antibody; (d) separating the solid carrier from unreacted second, labeled antibody; (e) measuring either the amount of second, labeled antibody associated with the solid carrier or the amount of unreacted second, labeled antibody; (f) relating the amount of measured second labeled antibody with the amount of labeled antibody measured for a control sample prepared in accordance with steps (a) thru (e), said control sample being known to be free of BAP, to determine the presence of BAP in said fluid sample, or relating the measured amount of labeled antibody for the fluid sample with the amount of labeled antibody measured for samples containing a known amount of BAP prepared in accordance with steps (a) thru (e) to determine the concentration of BAP in the fluid sample; wherein both monoclonal antibodies used are highly specific for BAP, especially in the presence of LAP.
IPC 1-7
C12N 5/12; C12P 21/08; G01N 33/543; G01N 33/573; G01N 33/577
IPC 8 full level
C12P 21/08 (2006.01); G01N 33/573 (2006.01); G01N 33/577 (2006.01); C12R 1/91 (2006.01)
CPC (source: EP US)
G01N 33/573 (2013.01 - EP US); Y10S 435/975 (2013.01 - EP); Y10S 436/808 (2013.01 - EP)
Designated contracting state (EPC)
AT BE CH DE DK ES FR GB GR IT LI LU NL SE
DOCDB simple family (publication)
EP 0381450 A2 19900808; EP 0381450 A3 19910918; EP 0381450 B1 19971105; AT E160021 T1 19971115; AU 4881890 A 19900809; AU 635518 B2 19930325; CA 2008304 A1 19900731; CA 2008304 C 20010327; DE 69031662 D1 19971211; DE 69031662 T2 19980305; DK 0381450 T3 19980727; ES 2110408 T3 19980216; GR 3025816 T3 19980331; JP 3055789 B2 20000626; JP H02259570 A 19901022; US 5525473 A 19960611; US 5589574 A 19961231
DOCDB simple family (application)
EP 90300959 A 19900130; AT 90300959 T 19900130; AU 4881890 A 19900125; CA 2008304 A 19900123; DE 69031662 T 19900130; DK 90300959 T 19900130; ES 90300959 T 19900130; GR 970403466 T 19971230; JP 2191390 A 19900130; US 23918594 A 19940505; US 45874095 A 19950602