EP 0381719 A1 19900816 - CELLULOSE BINDING FUSION PROTEINS.
Title (en)
CELLULOSE BINDING FUSION PROTEINS.
Title (de)
ZELLULOSEBINDENDE FUSIONSPROTEINE.
Title (fr)
PROTEINES DE FUSION SE LIANT A LA CELLULOSE.
Publication
Application
Priority
- GB 8900718 W 19890628
- US 21679488 A 19880708
Abstract (en)
[origin: US5202247A] A fusion protein is prepared containing a polypeptide such as an enzyme and an amino acid sequence having a substrate binding region of a polysaccharidase such as cellulase that has essentially no polysaccharidase activity. By contacting the fusion protein with an affinity matrix containing a substrate such as cellulose for the cellulase substrate binding region, the substrate binding region binds to the affinity matrix to immobilize the polypeptide. The polypeptide can be purified by separating the fusion protein or polypeptide from the affinity matrix. The polypeptide can be separated by cleaving the protein with a Cellulomonas fimi protease.
Abstract (fr)
Sont décrites de nouvelles compositions polypeptidiques et des procédés pour leur emploi, comprenant des protéines de fusion susceptibles de se lier à une matrice de polysaccharide. Ces compositions peuvent être synthétisées ou préparées par des techniques de recombinaison d'ADN.
IPC 1-7
IPC 8 full level
C12P 19/14 (2006.01); A61K 39/39 (2006.01); C07K 1/22 (2006.01); C07K 14/00 (2006.01); C07K 14/195 (2006.01); C07K 14/41 (2006.01); C07K 14/52 (2006.01); C07K 14/54 (2006.01); C07K 14/55 (2006.01); C07K 17/12 (2006.01); C07K 19/00 (2006.01); C08B 15/00 (2006.01); C08B 37/08 (2006.01); C08H 8/00 (2010.01); C12N 9/00 (2006.01); C12N 9/16 (2006.01); C12N 9/38 (2006.01); C12N 9/42 (2006.01); C12N 11/12 (2006.01); C12N 15/09 (2006.01); C12P 21/00 (2006.01); C12P 21/02 (2006.01); C12Q 1/56 (2006.01); D21H 17/00 (2006.01); G01N 33/531 (2006.01); C12R 1/19 (2006.01)
CPC (source: EP US)
A61K 39/39 (2013.01 - EP US); C07K 14/55 (2013.01 - EP US); C08B 15/00 (2013.01 - EP US); C08B 37/003 (2013.01 - EP US); C08H 8/00 (2013.01 - EP US); C12N 9/16 (2013.01 - EP US); C12N 9/2437 (2013.01 - EP US); C12N 9/2445 (2013.01 - EP US); C12N 9/2468 (2013.01 - EP US); C12N 11/12 (2013.01 - EP US); C12Q 1/56 (2013.01 - EP US); C12Y 302/01004 (2013.01 - EP US); C12Y 302/01021 (2013.01 - EP US); C12Y 302/01091 (2013.01 - EP US); D21H 17/005 (2013.01 - EP US); G01N 33/531 (2013.01 - EP US); C07K 2319/00 (2013.01 - EP US); C07K 2319/02 (2013.01 - EP US); C07K 2319/33 (2013.01 - EP US); C07K 2319/50 (2013.01 - EP US); C07K 2319/61 (2013.01 - EP US); C07K 2319/75 (2013.01 - EP US); Y10S 435/803 (2013.01 - EP US); Y10S 530/808 (2013.01 - EP US); Y10S 530/814 (2013.01 - EP US)
Designated contracting state (EPC)
AT BE CH DE FR GB IT LI LU NL SE
DOCDB simple family (publication)
WO 9000609 A1 19900125; AT E142699 T1 19960915; CA 1335182 C 19950411; DE 68927161 D1 19961017; DE 68927161 T2 19970130; EP 0381719 A1 19900816; EP 0381719 B1 19960911; JP 2528721 B2 19960828; JP H03501261 A 19910322; US 5137819 A 19920811; US 5202247 A 19930413
DOCDB simple family (application)
GB 8900718 W 19890628; AT 89907798 T 19890628; CA 603567 A 19890622; DE 68927161 T 19890628; EP 89907798 A 19890628; JP 50749689 A 19890628; US 21679488 A 19880708; US 60398790 A 19901025