EP 0393039 B1 19930707 - PROCESS FOR PRODUCING PEPTIDES BY SPECIFIC CLEAVAGE OF FUSION PROTEINS WITH COLLAGENASES OBTAINED BY GENETIC ENGINEERING
Title (en)
PROCESS FOR PRODUCING PEPTIDES BY SPECIFIC CLEAVAGE OF FUSION PROTEINS WITH COLLAGENASES OBTAINED BY GENETIC ENGINEERING
Publication
Application
Priority
DE 3731874 A 19870918
Abstract (en)
[origin: DE3731874A1] Fusion proteins are disclosed having the formula (I) H2N-Z1-X-(Pro-Y-Gly)n-Pro-Z2-COOH, in which n >/= 2, X and Y are each of the 20 amino-acids determined by the genetic code, Z1 is a bacterial amino-acid sequence and Z2 is the target peptide composed of any desired amino-acids of the genetic code. Also disclosed are their production process and their enzymatic cleavage to form desired eukaryotic proteins.
IPC 1-7
IPC 8 full level
C07K 14/00 (2006.01); C07K 14/195 (2006.01); C07K 14/41 (2006.01); C07K 14/575 (2006.01); C07K 14/695 (2006.01); C07K 19/00 (2006.01); C12N 15/09 (2006.01); C12N 15/62 (2006.01); C12N 15/66 (2006.01); C12P 21/02 (2006.01); C12P 21/06 (2006.01); C12R 1/19 (2006.01)
CPC (source: EP)
C07K 14/695 (2013.01); C12N 15/62 (2013.01); C12N 15/66 (2013.01); C07K 2319/00 (2013.01); C07K 2319/50 (2013.01)
Citation (examination)
Proc. Natl. Acad. Sci. USA, vol. 81, pp. 4692-4696, August 1984, J. Germino et al.: "Rapid purification of a cloned gene product by genetic fusion and site-specific proteolysis"
Designated contracting state (EPC)
AT BE CH DE FR GB IT LI LU NL SE
DOCDB simple family (publication)
DE 3731874 A1 19890330; EP 0393039 A1 19901024; EP 0393039 B1 19930707; JP H03500241 A 19910124; WO 8902461 A1 19890323
DOCDB simple family (application)
DE 3731874 A 19870918; DE 8800535 W 19880829; EP 88907257 A 19880829; JP 50695488 A 19880829