EP 0424633 A2 19910502 - Automated method and device for performing solid-phase diagnostic assay.
Title (en)
Automated method and device for performing solid-phase diagnostic assay.
Title (de)
Automatisierte Verfahren und Vorrichtung zur Ausführung von diagnostischen Festphasenanalysen.
Title (fr)
Procédé et appareil automatisés pour mener à bien des analyses diagnostiques en phase solide.
Publication
Application
Priority
US 42565189 A 19891023
Abstract (en)
A disposable device suitable for performing automated solid-phase diagnostic assays which employs microparticles to complex an analyte and where the microparticle complex becomes retained and immobilized on a fibrous matrix such that the presence of analyte on the microparticles can be detected by optical means. A device is disclosed having a sample well for receiving a sample and reagents for forming a reaction mixture, a read well comprising (a) an entrance port and holding means for receiving and holding a quantity of sample and assay reagents, (b) a fibrous matrix for retaining and immobilizing microparticle/analyte complexes for detection, said fibrous matrix positioned below said holding means, and having an average spatial separation of fibers greater than the average diameter of said microparticles, (c) means positioned below said fibrous matrix for assisting the flow of sample and assay reagents through said fibrous matrix, such as an absorbent material or vacuum below said fibrous matrix, (d) means for creating a substantially light-tight real surrounding the well, (e) means for venting air entrapped in the absorbent material and which is displaced by fluids absorbed therein, and (f) passage means communicating between the sample well and the read well whereby sample and reaction mixtures can be transferred and washed from said sample well into said read well without being contacted by any apparatus.
IPC 1-7
IPC 8 full level
B01L 3/00 (2006.01); C12M 1/34 (2006.01); G01N 21/78 (2006.01); G01N 33/543 (2006.01); G01N 33/576 (2006.01); G01N 33/94 (2006.01); G01N 35/02 (2006.01); G01N 35/10 (2006.01)
CPC (source: EP KR US)
B01L 3/50255 (2013.01 - EP US); G01N 33/53 (2013.01 - KR); G01N 33/54313 (2013.01 - EP US); G01N 33/54366 (2013.01 - EP US); G01N 33/5762 (2013.01 - EP US); G01N 33/94 (2013.01 - EP US); G01N 35/1002 (2013.01 - EP US); B01L 2300/069 (2013.01 - EP US); B01L 2300/0829 (2013.01 - EP US); B01L 2400/0406 (2013.01 - EP US); Y10S 435/805 (2013.01 - EP US); Y10T 436/255 (2015.01 - EP US); Y10T 436/2575 (2015.01 - EP US)
Designated contracting state (EPC)
AT BE CH DE ES FR GB IT LI NL
DOCDB simple family (publication)
EP 0424633 A2 19910502; EP 0424633 A3 19920923; EP 0424633 B1 19960117; AT E133263 T1 19960215; AU 5984890 A 19910426; AU 639703 B2 19930805; CA 2021587 A1 19910424; CA 2021587 C 20030408; DE 69024905 D1 19960229; DE 69024905 T2 19960905; ES 2084629 T3 19960516; JP 2515428 B2 19960710; JP H03160981 A 19910710; KR 910008408 A 19910531; US 5244630 A 19930914
DOCDB simple family (application)
EP 90116379 A 19900827; AT 90116379 T 19900827; AU 5984890 A 19900725; CA 2021587 A 19900719; DE 69024905 T 19900827; ES 90116379 T 19900827; JP 28323990 A 19901019; KR 900013589 A 19900831; US 42565189 A 19891023