EP 0468946 B1 19950712 - Enzymatic method of determining beta-lactam ring antibiotics.
Title (en)
Enzymatic method of determining beta-lactam ring antibiotics.
Title (de)
Enzymatisches Verfahren zur Bestimmung von beta-Laktam-Antibiotika.
Title (fr)
Procédé enzymatique pour la détermination d'antibiotiques à noyau beta-lactame.
Publication
Application
Priority
GB 9009692 A 19900430
Abstract (en)
[origin: EP0468946A1] It entails the following stages: 1) incubation of the biological fluid with the soluble D-alanyl-D-alanine carboxypeptidase produced by the strain Actinomadura R39; 2) incubation of the mixture obtained with a solution of substrate, which is a thioester of formula <IMAGE> in the presence of an amino acid of D configuration or glycine; 3) determination of the quantity of 2-mercaptoalkanoic acid of formula <IMAGE> formed in stage 2); 4) comparison of the determination of stage 3) with a standard. <??>In these formulae: R1 = benzoyl, phenylacetyl or Nalpha-acetyl-Llysyl, R2 = glycyl or D-alanyl, and R3 = H or CH3. <??>The invention also relates to a test kit comprising the reagents to be employed for implementation of the method.
IPC 1-7
IPC 8 full level
C07K 5/068 (2006.01); C07K 14/505 (2006.01); C12Q 1/37 (2006.01); A61K 38/00 (2006.01); C12R 1/03 (2006.01)
CPC (source: EP US)
C07K 5/06086 (2013.01 - EP US); C12Q 1/37 (2013.01 - EP US); A61K 38/00 (2013.01 - EP US); Y10S 435/81 (2013.01 - EP US); Y10S 435/825 (2013.01 - EP US); Y10S 435/975 (2013.01 - EP US); Y10S 436/815 (2013.01 - EP US)
Designated contracting state (EPC)
AT BE CH DE DK ES FR GB GR IT LI LU NL SE
DOCDB simple family (publication)
EP 0468946 A1 19920129; EP 0468946 B1 19950712; AT E124998 T1 19950715; AU 636482 B2 19930429; AU 7622691 A 19911107; BG 61082 B2 19961031; CA 2040180 A1 19911031; CA 2040180 C 20010619; CS 9101246 A2 19911112; CZ 280282 B6 19951213; DE 69111149 D1 19950817; DE 69111149 T2 19951207; DK 0468946 T3 19951009; ES 2075414 T3 19951001; FI 912054 A0 19910429; FI 912054 A 19911031; FI 97151 B 19960715; FI 97151 C 19961025; GB 9009692 D0 19900620; GR 3017235 T3 19951130; HU 214926 B 19980728; HU 911441 D0 19911128; HU T58825 A 19920330; IE 69037 B1 19960807; IE 911433 A1 19911106; JP 2823382 B2 19981111; JP H0686695 A 19940329; NO 302664 B1 19980406; NO 911692 D0 19910429; NO 911692 L 19911031; NZ 237950 A 19920225; PL 169360 B1 19960731; PL 290061 A1 19911202; PT 97511 A 19920131; PT 97511 B 19980831; SK 279176 B6 19980708; US 5246830 A 19930921; YU 48050 B 19961018; YU 78391 A 19940120
DOCDB simple family (application)
EP 91870070 A 19910426; AT 91870070 T 19910426; AU 7622691 A 19910426; BG 9432791 A 19910429; CA 2040180 A 19910410; CS 124691 A 19910430; DE 69111149 T 19910426; DK 91870070 T 19910426; ES 91870070 T 19910426; FI 912054 A 19910429; GB 9009692 A 19900430; GR 950402344 T 19950830; HU 144191 A 19910429; IE 143391 A 19910429; JP 9676291 A 19910426; NO 911692 A 19910429; NZ 23795091 A 19910426; PL 29006191 A 19910426; PT 9751191 A 19910429; SK 124691 A 19910430; US 69235591 A 19910426; YU 78391 A 19910430