Title (en)

RECOMBINANT THYROTROPIN RECEPTOR

Publication

EP 0506890 A4 19930630 (EN)

Application

EP 91904350 A 19901219

Priority

• US 45197389 A 19891220
• US 57501890 A 19900830

Abstract (en)

[origin: WO9109137A1] Functionally-active, human thyrotropin receptor has been expressed in non-thyroidal eukaryotic cells. A human thyroid cDNA library was screened with two synthetic oligonucleotides based on the reported amino acid sequence of the 3rd and 4th transmembrane domains of a putative human thyrotropin receptor and related receptors. The nucleotide sequence of a 4 kb clone revealed an open reading frame of 764 amino acids (86,816 Daltons) with a putative signal peptide, seven transmembrane domains, five potential glycosylation sites, and a very short intracytoplasmic region. Homology with the extracellular domain of the pig LH/CG receptor was only 33 %. Chinese hamster ovary cells stably transfected with this cDNA in an expression vector generated a functional receptor, able to activate adenylate cyclase, specifically in response to thyrotropin stimulation.

IPC 1-7

C12Q 1/00; C12Q 1/68; G01N 33/53

IPC 8 full level

A61K 38/00 (2006.01); A61K 39/395 (2006.01); A61P 5/12 (2006.01); A61P 37/00 (2006.01); C07K 14/00 (2006.01); C07K 14/705 (2006.01); C07K 14/72 (2006.01); C07K 16/00 (2006.01); C07K 16/28 (2006.01); C12N 5/10 (2006.01); C12N 15/09 (2006.01); C12P 21/02 (2006.01); C12P 21/08 (2006.01); G01N 33/53 (2006.01); G01N 33/564 (2006.01); G01N 33/76 (2006.01); C12R 1/91 (2006.01)

CPC (source: EP)

A61P 5/12 (2017.12); A61P 37/00 (2017.12); C07K 14/723 (2013.01); C07K 16/2869 (2013.01); G01N 33/564 (2013.01); G01N 33/76 (2013.01); A61K 38/00 (2013.01); C07K 2319/00 (2013.01); C07K 2319/33 (2013.01)

Citation (search report)

• WO 8402779 A1 19840719 - BETH ISRAEL HOSPITAL [US]
• EP 0433509 A2 19910626 - HENNING BERLIN GMBH [DE]
• WO 9013643 A2 19901115 - GENENTECH INC [US]
• WO 9208726 A1 19920529 - AKAMIZU TAKASHI [JP], et al
• WO 9103483 A1 19910321 - NEW ENGLAND MEDICAL CENTER INC [US]
• ENDOCRINOLOGY vol. 110, no. 4, 1982, THE ENDOCRINE SOC., US pages 1381 - 1391 Koizumi, Y.; Zakarija, M.; McKenzie, J. M. 'Solubilization, purification, and partial characterization of thyrotropin receptor from bovine and human thyroid glands'
• 64th Meeting of the American Thyroid ass.; 1989; abstract T-51; Frazier-Seabrook et al.; "Isolation of a thyroid-specific cDNA with over 80% homology with the luteinizing hormone receptor."
• CLINICAL RESEARCH vol. 29, no. 2, April 1981, NEW YORK, US page 491A ISLAM, M.N. ET AL. 'Purification of the human thyrotropin receptor'
• luteinizing hormone receptor."
• CLINICAL RESEARCH vol. 29, no. 2, April 1981, NEW YORK, US page 294A ISLAM, M.N. ET AL. 'Purification of the human thyrotropin receptor'
• CLINICAL CHEMISTRY vol. 34, no. 8, 1988, NEW YORK, US page 1662 Tamaki, H. et al.; 'On standardization of the radioreceptor assay for anti-thyrotropin receptor antibody.'
• ACTA ENDOCRINOLOGICA vol. 115, 1987, pages 166 - 172 Chan, J. et al.; 'TSH receptor structure.'
• PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF USA. vol. 79, November 1982, WASHINGTON US pages 6680 - 6684 Valente et al.; 'Monoclonal antibodies to the thyrotropin receptor : Stimulating and blocking antibodies derived from the lymphocytes of patients with Graves disease.'
• See references of WO 9109137A1

Designated contracting state (EPC)

AT BE CH DE DK ES FR GB GR IT LI LU NL SE

DOCDB simple family (publication)

WO 9109137 A1 19910627; AU 7257391 A 19910718; EP 0506890 A1 19921007; EP 0506890 A4 19930630; IE 904641 A1 19910717; JP H05504683 A 19930722

DOCDB simple family (application)

US 9007387 W 19901219; AU 7257391 A 19901219; EP 91904350 A 19901219; IE 464190 A 19901220; JP 50433290 A 19901219