EP 0537299 A4 19930205 - OLIGONUCLEOTIDE-TRANSPORT AGENT DISULFIDE CONJUGATES.
Title (en)
OLIGONUCLEOTIDE-TRANSPORT AGENT DISULFIDE CONJUGATES.
Title (de)
DISULFIDKONJUGATE VON OLIGONUKLEOTID-TRANSPORTAGENTEN.
Title (fr)
CONJUGUES A BASE DE DISULFURE D'OLIGONUCLEOTIDE ET D'UN AGENT DE TRANSPORT.
Publication
Application
Priority
US 50236190 A 19900329
Abstract (en)
[origin: WO9114696A1] The invention relates to compositions and methods for enhancing the delivery of an oligonucleotide into a cell. The compositions of the invention comprise oligonucleotide conjugates which consist of an oligonucleotide, conjugated via a molecular linker containing at least one disulfide bond, to an agent (termed herein "transport agent") which facilitates transport across an outer cell membrane, or across the blood-brain barrier. In a preferred aspect, the disulfide linkage is cleaved upon uptake of the composition by the cell. Pharmaceutical compositions comprising an oligonucleotide conjugate of the invention may be used to treat a wide variety of diseases and disorders. Methods for inhibiting the expression of a nucleic acid sequence within a cell, and methods for detecting a nucleic acid sequence within a cell are also provided. In a specific embodiment, an oligonucleotide conjugated to cholesterol via a linker containing a disulfide linkage can be used for therapeutic or diagnostic purposes, by hybridization of the oligonucleotide to a complementary nucleic acid sequence in a procaryotic or eucaryotic cell.
IPC 1-7
IPC 8 full level
A61K 31/70 (2006.01); A61K 48/00 (2006.01); C07H 21/04 (2006.01); C12N 15/87 (2006.01); C12Q 1/68 (2006.01)
CPC (source: EP)
A61K 47/554 (2017.07); C12N 15/87 (2013.01); C12Q 1/6813 (2013.01); C12Q 1/6841 (2013.01)
Citation (search report)
No further relevant documents have been disclosed.
Designated contracting state (EPC)
DE FR GB
DOCDB simple family (publication)
WO 9114696 A1 19911003; AU 7759291 A 19911021; CA 2079109 A1 19910930; EP 0537299 A1 19930421; EP 0537299 A4 19930205; JP H05505941 A 19930902
DOCDB simple family (application)
US 9102224 W 19910329; AU 7759291 A 19910329; CA 2079109 A 19910329; EP 91918074 A 19910329; JP 50858691 A 19910329