Global Patent Index - EP 0540575 A4

EP 0540575 A4 19940608 - HUMAN MEG-CSF PROTEIN AND METHODS

Title (en)

HUMAN MEG-CSF PROTEIN AND METHODS

Publication

EP 0540575 A4 19940608 (EN)

Application

EP 91913186 A 19910702

Priority

US 54757390 A 19900702

Abstract (en)

[origin: WO9200319A1] An isolated human megakaryocyte-colony stimulating factor (hMeg-CSF protein) preferably purified to homogeneity from urine of aplastic anemia patients is disclosed. The novel hMeg-CSF protein is a basic protein having a pI equal to about 7.2-7.4 as determined by isoelectric focusing and a molecular weight of about 29.000-34.000 daltons as determined by SDS-PAGE when in a glycosylated and sialyated form. The novel hMeg-CSF protein has the ability to induce the formation megakaryocyte-colony forming units in a murine fibrin clot assay in vitro, and the further ability to regulate megakaryocytopoiesis and platelet production in vivo. The hMeg-CSF protein can be further characterized as a glycoprotein having biantennary carbohydrate structures, beta-galactose residues as terminal or penultimate sugars and sialic acid moieties. The hMeg-CSF protein is believed to have a specific activity of at least about 4.000 CFU-Meg colonies/mg protein in murine fibrin clot assay. Unique pharmaceuticals containing the hMeg-CSF protein for treating animals including humans and methods for isolating the novel hMeg-CSF protein at various levels of purity are also disclosed.

IPC 1-7

C12N 15/27

IPC 8 full level

A61K 38/00 (2006.01); A61P 7/00 (2006.01); C07K 1/16 (2006.01); C07K 1/18 (2006.01); C07K 1/20 (2006.01); C07K 1/26 (2006.01); C07K 14/435 (2006.01); C07K 14/52 (2006.01); C07K 14/53 (2006.01); C12P 21/02 (2006.01)

CPC (source: EP KR)

A61P 7/00 (2017.12 - EP); C07K 1/00 (2013.01 - KR); C07K 14/53 (2013.01 - EP); A61K 38/00 (2013.01 - EP)

Citation (search report)

  • [DPX] WO 9102001 A1 19910221 - GENETICS INST [US]
  • [X] WO 9003397 A1 19900405 - MEIJI SEIKA KAISHA [JP] & EP 0516845 A1 19921209 - MEIJI SEIKA KAISHA [JP]
  • [DA] K. OGATA ET AL: "Partial purification and characterization of human Megakaryocyte colony-stimulating factor ( Meg-CSF )", INTERNATIONAL JOURNAL OF CELL CLONING, vol. 8, 1990, pages 103 - 120, XP000917130 & INTERNATIONAL CAPRI CONFERENCE, OCTOBER 6-12 , 1989
  • [DX] SHIMIZU T. ET AL: "Purification of human megakaryocyte colony-stimulating factor", EXPERIMENTAL HEMATOLOGY, vol. 14, no. 6, July 1986 (1986-07-01), pages 490
  • [T] ZHANG Z. G. ET AL: "Further purification of megakaryocyte colony stimulating factor ( Meg-CSF ) from the urine of patients with aplastic anemia", EXPERIMENTAL HEMATOLOGY, vol. 19, no. 6, 21 July 1991 (1991-07-21), pages 493
  • [X] SHIMIZU T. ET AL: "purification of human sialylated megakaryocyte colony-stimulating factor", EXPERIMENTAL HEMATOLOGY, vol. 15, no. 5, August 1987 (1987-08-01), pages 492, XP000645923
  • See references of WO 9200319A1

Designated contracting state (EPC)

AT BE CH DE DK ES FR GB GR IT LI LU NL SE

DOCDB simple family (publication)

WO 9200319 A1 19920109; AU 8215591 A 19920123; CA 2086248 A1 19920103; EP 0540575 A1 19930512; EP 0540575 A4 19940608; FI 925983 A0 19921231; FI 925983 A 19921231; JP H06502621 A 19940324; KR 930701475 A 19930611

DOCDB simple family (application)

US 9104698 W 19910702; AU 8215591 A 19910702; CA 2086248 A 19910702; EP 91913186 A 19910702; FI 925983 A 19921231; JP 51292191 A 19910702; KR 920703438 A 19921231