EP 0542874 A4 19940511 - CIRCULAR EXTENSION FOR GENERATING MULTIPLE NUCLEIC ACID COMPLEMENTS
Title (en)
CIRCULAR EXTENSION FOR GENERATING MULTIPLE NUCLEIC ACID COMPLEMENTS
Publication
Application
Priority
US 55864590 A 19900725
Abstract (en)
[origin: WO9201813A1] A process for generating multiple linear complements of a single strand, circular nucleic acid template containing at least one cleavage site is described. The process consists of combining the single strand, circular nucleic acid template with polynucleotide primers under conditions sufficient for hybridization; extending the polynucleotide primer more than once around the circle to generate a complementary displacement of more than one contiguous complement of the single strand, circular nucleic acid template. Also described is a process of synthesizing novel single strand, circular nucleic acids between 30 and 2200 nucleotides. The process consists of synthesizing a linear polynucleotide; combining the linear polynucleotide with a complementary linking oligonucleotide under conditions sufficient for hybridization; and ligating the linear polynucleotide to produce a single strand, circular nucleic acid.
IPC 1-7
IPC 8 full level
C12N 15/09 (2006.01); C12N 15/10 (2006.01); C12Q 1/68 (2006.01)
CPC (source: EP)
C12N 15/10 (2013.01); C12Q 1/6844 (2013.01)
Citation (search report)
- [X] WO 8909824 A2 19891019 - BRITISH BIO TECHNOLOGY [GB]
- [X] EP 0310030 A1 19890405 - SANWA KAGAKU KENKYUSHO CO [JP]
- [PXPY] WO 9106643 A1 19910516 - CANADA MAJESTY IN RIGHT OF [CA]
- [PY] EP 0395398 A2 19901031 - LIFE TECHNOLOGIES INC [US]
- [PXPY] US 4994370 A 19910219 - SILVER JONATHAN [US], et al
- [E] WO 9116446 A1 19911031 - CONSEJO SUPERIOR INVESTIGACION [ES], et al
- [Y] BENJAMIN LEWIN, GENES, 1983, JOHN WILEY & SONS, NEW YORK, US;, pages 515 - 518
- [T] A. KORNBERG AND T.A. BAKER, DNA REPLICATION, 2ND EDITION, 1992, W.H. FREEMAN AND COMPANY, NEW YORK, US;, pages 502 - 503
- See references of WO 9201813A1
Designated contracting state (EPC)
AT BE CH DE DK ES FR GB GR IT LI LU NL SE
DOCDB simple family (publication)
WO 9201813 A1 19920206; AU 649066 B2 19940512; AU 8417391 A 19920218; CA 2087256 A1 19920126; EP 0542874 A1 19930526; EP 0542874 A4 19940511; JP H06500014 A 19940106
DOCDB simple family (application)
US 9105067 W 19910717; AU 8417391 A 19910717; CA 2087256 A 19910717; EP 91915255 A 19910717; JP 51373191 A 19910717