EP 0546084 A4 19941019 - PROTEASES CAUSING ABNORMAL DEGRADATION OF AMYLOID -G(B)-PROTEIN PRECURSOR
Title (en)
PROTEASES CAUSING ABNORMAL DEGRADATION OF AMYLOID -G(B)-PROTEIN PRECURSOR
Publication
Application
Priority
US 56880690 A 19900817
Abstract (en)
[origin: WO9203542A1] A proteolytic factor is capable of cleaving beta -protein precursor at a site near the beta -protein N-terminus. Also, a method for treating Alzheimer's disease in a patient includes steps of reducing DOLLAR (b)-protein precursor proteolysis outside the beta -protein domain at a site near the beta -protein N-terminus. Also, a method for purifying en enzyme from a sample includes steps of incubating the sample with a labelled substrate of the enzyme or with a labelled fragment of a substrate to which the enzyme binds, treating the sample with a crosslinking agent to crosslink any enzyme-substrate complexes in the sample, and recovering labelled complexes. Also, a method for diagnosis in a subject of a disease characterized by accumulation of amyloid includes determining the level, in a sample of tissue or body fluid from the subject, of an AD proteolytic factor. Also, a method for screening for an agent useful in treatment of a disease characterized by accumulation of amyloid includes steps of incubating an AD protease with a peptide having an amino acid sequence corresponding to the sequence spanning the beta -protein N-terminus in the presence of a candidate agent, and determining the degradation of the peptide.
IPC 1-7
IPC 8 full level
A61K 38/55 (2006.01); A61P 25/28 (2006.01); C07K 14/47 (2006.01); C12N 9/64 (2006.01); G01N 33/15 (2006.01); G01N 33/50 (2006.01)
CPC (source: EP)
A61K 38/55 (2013.01); A61P 25/28 (2017.12); C07K 14/4711 (2013.01); C12N 9/6472 (2013.01)
Citation (search report)
- [X] T. MIYATAKE ET AL: "Molecular biology and genetics of Alzheimer's disease. Proceedings of the International Symposium on Dementia: Niigate, Japan, 11-14 November 1989", EXCERPTA MEDICA, AMSTERDAM NL
- [X] J. NAVTIG ET AL: "Amyloid and Amyloidosis,1990; VIth International Symposium on Amyloidosis, Oslo, Norway, August 5-8, 1990", KLUWER, DORDRECHT NL
- [X] C. ABRAHAM ET AL: "Studies on a brain protease thet cleaves at the N-terminus of beta-protein related synthetic peptides", NEUROBIOLOGY OF AGING, vol. 11, June 1990 (1990-06-01), pages 303
- [X] R. NELSON AND H. POTTER: "Purification of clipsin, an alpha1-antichymotrypsin-binding protease which cleaves the beta-protein precursor", NEUROBIOLOGY OF AGING, vol. 11, June 1990 (1990-06-01), pages 303
- See references of WO 9203542A1
Designated contracting state (EPC)
AT BE CH DE DK ES FR GB GR IT LI LU NL SE
DOCDB simple family (publication)
WO 9203542 A1 19920305; AU 643835 B2 19931125; AU 8538791 A 19920317; EP 0546084 A1 19930616; EP 0546084 A4 19941019; HU 9300421 D0 19931129; HU T69771 A 19950928; JP H06503948 A 19940512
DOCDB simple family (application)
US 9105932 W 19910819; AU 8538791 A 19910819; EP 91916872 A 19910819; HU 42193 A 19910819; JP 51532791 A 19910819