Global Patent Index - EP 0548056 B2

EP 0548056 B2 2001-04-04 - A process for cloning methylated DNA in a bacterial host incapable of host restriction

Title (en)

A process for cloning methylated DNA in a bacterial host incapable of host restriction

Title (de)

Klonierung von methylierter DNS in einem bakteriellen Wirtsstamm der zur Wirtsrestriktion unfähig ist

Title (fr)

Processus pour le clonage d'ADN méthylée dans une souche bactérienne hôte qui est incapable de restriction

Publication

EP 0548056 B2 (EN)

Application

EP 93200367 A

Priority

  • EP 89201895 A
  • NL 8801826 A

Abstract (en)

[origin: EP0353812A2] A process for the rescue and cloning of a DNA sequence wherein a vector containing said DNA sequence is multiplied in a suitable bacterial host. As the bacterial host, a bacterial strain which is incapable of host restriction is used, such as an Escherichia coli C strain. Preferably, a bacteriophage vector which is a suitable cloning vehicle for the bacterial host concerned is used as the vector, the step of in vitro packaging into phage coats being performed by means of a phage packaging extract obtained from a bacterial strain which is incapable of host restriction. The above may be used in a process for detecting mutations in one or more marker genes introduced into a mammalian genome by means of a vector, which process comprises isolating DNA from cells of the transgenic mammal or from the mammalian cells, recovering the vector from said DNA, multiplying the vector in a suitable bacterial host which is deficient with respect to at least one of the marker genes, and testing marker gene expression. Preferably, the DNA isolated from cells of the transgenic mammal or the mammalian cells is pre-purified by fragmenting the DNA by means of a restriction enzyme which does not have a cutting site within the vector, separating the resulting fragments on the basis of a suitable criterion, such as differences in size, and collecting fragments comprising the vector, whereafter the vector is recovered from said pre-purified DNA.

IPC 1-7 (main, further and additional classification)

C12N 15/00; C12Q 1/68; C12N 15/10; C12N 15/85; C12N 15/73

IPC 8 full level (invention and additional information)

A01K 67/027 (2006.01); C12N 1/21 (2006.01); C12N 15/09 (2006.01); C12N 15/10 (2006.01); C12N 15/73 (2006.01); C12N 15/85 (2006.01); C12Q 1/68 (2006.01); C12R 1/19 (2006.01)

CPC (invention and additional information)

C12N 15/73 (2013.01); C12N 15/10 (2013.01); C12N 15/85 (2013.01); C12Q 1/68 (2013.01); C12Q 1/6827 (2013.01)

Citation (opposition)

Opponent :

  • Raleigh. Restriktion and modification in vivo by Escherichia coli K12. Methods in Enzymology 12, 130-141 (1987)
  • Rosenberg et al. Improved in vitro packaging of coliphage lambda DNA; a one strain system free from endogenous phage. Gene 38, 165-175 (1985)
  • Rosenberg. EcoK restriction cloning during in vitro packaging of coliphage Lambda DNA. Gene 39, 313-315 (1985)

Designated contracting state (EPC)

AT BE CH DE ES FR GB GR IT LI LU NL SE

EPO simple patent family

EP 0353812 A2 19900207; EP 0353812 A3 19900307; EP 0353812 B1 19940112; AT 100151 T 19940115; AT 132529 T 19960115; DE 68912228 D1 19940224; DE 68912228 T2 19940804; DE 68925369 D1 19960215; DE 68925369 T2 19960515; DE 68925369 T3 20010809; EP 0548056 A1 19930623; EP 0548056 B1 19960103; EP 0548056 B2 20010404; EP 0683238 A2 19951122; EP 0683238 A3 19951220; ES 2061943 T3 19941216; ES 2081679 T3 19960316; GR 3018927 T3 19960531; JP H02142471 A 19900531; JP H0829093 B2 19960327; NL 8801826 A 19900216; US 5470706 A 19951128

INPADOC legal status


2009-06-30 [PG25 ES] LAPSED IN A CONTRACTING STATE ANNOUNCED VIA POSTGRANT INFORM. FROM NAT. OFFICE TO EPO

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2001-04-04 [XX] MISCELLANEOUS:

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1999-04-14 [R26] OPPOSITION FILED (CORRECTION)

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- Effective date: 19961002

1997-05-30 [REG FR ST] NOTIFICATION OF LAPSE

1997-04-07 [EUG] SE: EUROPEAN PATENT HAS LAPSED

1997-04-01 [NLV4] NL: LAPSED OR ANULLED DUE TO NON-PAYMENT OF THE ANNUAL FEE

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1997-02-28 [REG GR MM2A] ANNULMENT OR LAPSE DUE TO NON-PAYMENT OF FEES

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1997-02-03 [NLR1] NL: OPPOSITION HAS BEEN FILED WITH THE EPO

- Opponent name: BOEHRINGER MANNHEIM GMBH PATENTABTEILUNG

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1996-12-04 [26] OPPOSITION FILED

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1996-03-16 [REG ES FG2A] DEFINITIVE PROTECTION

- Document: ES 2081679 T3

1996-03-15 [REG CH NV] NEW AGENT

- Representative's name: ARNOLD & SIEDSMA AG

1996-02-15 [REF] CORRESPONDS TO:

- Document: DE 68925369 19960215

1996-01-24 [ITF] IT: TRANSLATION FOR A EP PATENT FILED

- Owner name: STUDIO INGG. FISCHETTI & WEBER

1996-01-03 [AC] DIVISIONAL APPLICATION (ART. 76) OF:

- Document: EP 353812

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- Document: AT 132529 T 19960115

1996-01-03 [XX] MISCELLANEOUS:

- Free text: TEILANMELDUNG 95201753.1 EINGEREICHT AM 18/07/89.

1993-12-15 [17Q] FIRST EXAMINATION REPORT

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