EP 0557350 B1 19941005 - ASSAY FOR GLYCATED BLOOD PROTEINS.
Title (en)
ASSAY FOR GLYCATED BLOOD PROTEINS.
Title (de)
BESTIMMUNG VON GLYCOSILIERTEN BLUTPROTEINEN.
Title (fr)
ANALYSE POUR PROTEINES DU SANG GLUCOSEES.
Publication
Application
Priority
- EP 9102163 W 19911113
- GB 9024771 A 19901114
Abstract (en)
[origin: WO9208984A1] There is provided a method of assessing a glycated blood protein in a sample, said method comprising the steps of a) optionally hemolysing said sample to liberate cell bound glycated protein; b) separating said glycated blood protein and the corresponding non-glycated blood protein from said sample; c) contacting said sample before or during separation of said glycated and non-glycated proteins therefrom or contacting said separated proteins with a first signal forming agent capable of binding to said glycated protein but not to the corresponding non-glycated protein; d) optionally, contacting said sample before or during separation of said glycated and non-glycated proteins therefrom or contacting said separated proteins with a second signal forming agent capable of binding to said glycated protein and to said corresponding non-glycated protein; and e) assessing the signal forming agents which have bound to said separated proteins and/or which have not bound to said glycated protein or said corresponding non-glycated protein; with the proviso that where said glycated protein comprises glycated hemoglobin said first signal forming agent is a chromophore labelled boronic acid or salt thereof having an absorption maximum at above 600 nm. In this method the glycated protein which is labelled by the first signal forming agent may be any one, or indeed any set, of the blood proteins. By appropriate selection of the proteins under assay an indication may be obtained of the history of the patient's blood glucose control over the short, medium and long (up to 3 months) term.
IPC 1-7
IPC 8 full level
G01N 21/31 (2006.01); G01N 30/88 (2006.01); G01N 33/68 (2006.01); G01N 33/72 (2006.01)
CPC (source: EP US)
G01N 33/68 (2013.01 - EP US); G01N 33/6842 (2013.01 - EP US); G01N 33/723 (2013.01 - EP US); Y10S 436/815 (2013.01 - EP US); Y10S 436/824 (2013.01 - EP US); Y10T 436/25125 (2015.01 - EP US); Y10T 436/25375 (2015.01 - EP US)
Designated contracting state (EPC)
AT BE CH DE DK ES FR GB GR IT LI LU NL SE
DOCDB simple family (publication)
WO 9208984 A1 19920529; AT E112635 T1 19941015; AU 8866291 A 19920611; CA 2096250 A1 19920515; CA 2096250 C 20020924; DE 69104491 D1 19941110; DE 69104491 T2 19950216; DK 0557350 T3 19941107; EP 0557350 A1 19930901; EP 0557350 B1 19941005; ES 2061273 T3 19941201; FI 104519 B 20000215; FI 932150 A0 19930512; FI 932150 A 19930603; GB 9024771 D0 19910102; JP 2643027 B2 19970820; JP H06502244 A 19940310; NO 311385 B1 20011119; NO 931747 D0 19930513; NO 931747 L 19930714; US 5506144 A 19960409; US 5919708 A 19990706
DOCDB simple family (application)
EP 9102163 W 19911113; AT 91919886 T 19911113; AU 8866291 A 19911113; CA 2096250 A 19911113; DE 69104491 T 19911113; DK 91919886 T 19911113; EP 91919886 A 19911113; ES 91919886 T 19911113; FI 932150 A 19930512; GB 9024771 A 19901114; JP 51799191 A 19911113; NO 931747 A 19930513; US 5027493 A 19930712; US 57056995 A 19951211